HUVE cells were plated at low density (1000 cells/well) into 96-multiwell plates in M199 complete medium and treated with increasing dilutions of A009, hydroxytyrosol alone (HT) or ethanol (data not shown). Cell proliferation was evaluated at determined times (24, 48,72, 96h) using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. A 009 inhibited cell proliferation in a dose-dependent manner; the inhibitory effect of A009 was already significant after 24h of treatment with a dose of 1:500, whereas HT alone showed no significant inhibitory effect (A). We calculated the IC50 treatment in order to define the effectiveness of A009 and HT in inhibiting HUVE cells proliferation. A009 showed lower IC50 value as compared to HT alone, demonstrating the grater effectiveness of the purified extract (B).
Mean ± SD of 3 independent experiments is shown. Student' s t-test was used to determine pvalue (***p<0.001; ****p<0.0001).
