Figure 3: Anti-oxidative effects of A009
The ability of A009 to interfere with the induction of reactive oxygen species (ROS) was assessed by staining with DCFH-DA followed by flow cytometry analysis. HUVECs (1.5 x 105 cells/treatment) were pre-treated with decreasing dilution (1:500 and 1:250) of A009, HT and EtOH, then followed by exposure to 250μM H2O2(A) or pretreated with 250μM H2O2 followed by treatment with decreasing dilution (1:500 and 1:250) of A009, HT and EtOH (B), to assess the scavenger activity in a prevention and an intervention schedule, respectively. Fluorescence was measured with FACsCanto and data analyzed with the FACSDiva Software 6.1.2. A009 was able to inhibit the ROS production in both schedules in a dose-dependent manner with more efficacy than HT alone. Complete medium (NT, medium M199 supplemented with 10% FBS) and H2O2 (250μM) were used as negative and positive control, respectively. Mean ± SD of 3 independent experiments is shown. Student's t-test was used to determine p-value. (**p<0.01;***p<0.001).
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