The ability of A009 to interfere with HUVEC migration (A) and invasion (B) were assessed using a modified Boyden chamber assays. HUVECs were pretreated for 24h with decreasing dilutions of A009, HT or EtOH (1:500-1:250), then the HUVECs were seeded in the upper compartment of Boyden chamber in serum-free medium. Complete medium (M199 supplemented with 10% FBS) or serum-free medium was placed in the lower compartment.
Following 6 or 24 hours of incubation for the assessment of migration or invasion, respectively, the migrated/invaded cells were fixed, stained and counted. A009 was able to inhibit migration and invasion at lower concentrations as compared to HT alone. Mean ± SD of 3 independent experiments is shown. Student’ s t-test was used to determine p-value (***p<0.001; ****p<0.0001) for migration and (*p<0.05; **p<0.01) for invasion.
