Figure 6: Anti-angiogenic activities of A009 in vivo.
Matrigel containing a pro angiogenic mixture (VTH: VEGF, TNF-α, heparin), with or without either A009 or HT alone, were subcutaneously injected into the flanks of mice. The Matrigel pellets were excised 4 days after injection, recovered, observed for macroscopic red coloration (A) and assessed for hemoglobin (Hb) content using the colorimetric Drabkin’s assay (B). Hematoxylin and eosin staining of explanted matrigel pellets (C) were performed to evaluate the presence of total cell infiltrate. A009 was more effective than HT alone in inhibiting the angiogenic process. Mean ± SD is shown, (*p<0.05; **p<0.01).