Figure 6: Calculated absolute amount of dystrophin in muscle biopsies obtained from BMD and age matched healthy controls.
Total protein extract (50 μg) from each biopsy was spiked with 30 μg of 13C6-Lysine labeled SILAC mouse muscle extract and processed using the pre- digestion spike-in approach (see Figure 1 and Methods). Intensity ratios of labeled and unlabeled peptide pairs were used to determine the absolute amount of dystrophin in each muscle extract.
Goto home»