The relative quantity of SK3 variant 3 did not differ between myometrium from pregnant and non-pregnant women (P=0.332). Variant 2 was not found present at detectable amounts. In contrast, SK3 variant 1 showed a 3-fold down-regulation in pregnant compared to non-pregnant myometrium (P=0.002). CyPPA depressed spontaneous phasic contractions in human myometrial strips of both non-pregnant and pregnant origin. It took place in a concentration-dependent manner (where pIC50 = 5.02 ± 0.08 and 5.16 ± 0.15, respectively, and P>0.05), with contractions being abolished at 60 μM CyPPA.
Citation: Rosenbaum ST, Svalo J, Larsen T, Joergensen J, Bouchelouche P (2013) Relaxant Effect of Cyppa In Vitro and Expression Analysis of SK3 Splice Variants in the Myometrium of Pregnant and Non-Pregnant Women. Gynecol Obstet 3:171. doi: 10.4172/2161-0932.1000171