We used a relaxed-clock program (Multidivtime) to estimate the phylogenetic relatedness and substitution rates by comparative analyses of 16S rRNA gene and ITS region sequences. Four rhizobial strains isolated from three Tunisian wild Sulla legumes were used together with reference strains to obtain the 16S rRNA gene and ITS region alignments. Bayesian inferred trees were congruent, and showed a clear split between Agrobacterium and Rhizobium species. Among the four Tunisian isolates, Hsc01 belonged to Rhizobium species and formed a monophyletic clade with Rhizobium sullae.
Citation: Chriki-Adeeb R, Chriki A (2015) Bayesian Phylogenetic Analysis of Rhizobia Isolated From Root-Nodules of ThreeTunisian Wild Legume Species of the Genus Sulla. J Phylogen Evolution Biol 3:149. doi: 10.4172/2329-9002.1000149