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Volume 8

Journal of Gastrointestinal & Digestive System

ISSN: 2161-069X

Bariatric Surgery 2018 & Gastro 2018

March 15-16, 2018

JOINT EVENT

12

th

Global Gastroenterologists Meeting

3

rd

International Conference on Metabolic and Bariatric Surgery

&

March 15-16, 2018 Barcelona, Spain

CrosstalkofmTOR/HIF-1a/PKM2 andSTAT3/C-MYCsignalingpathways regulate the energymetabolism

and acidic microenvironment of gastric cancer

Chen Min, Sumeng Gao

and

Xiaoping Zou

The Affiliated Drum Tower Hospital - Nanjing University Medical School, P R China

C

ancer cells consume large amounts of glucose to produce lactate, even in the presence of ample oxygen. This phenomenon is

called the Warburg effect. C-Myc is an important member of the Myc gene family and is involved in the development of various

tumors. It plays an important role in the regulation of tumor energy metabolism which can regulate glycolysis to promote tumor

Warburg effect. Our study aims to improve the malignant biological behavior by controlling the energy metabolism of gastric cancer

through the mTOR/HIF-1α/PKM2 and STAT3/c-Myc signaling pathway through a series experiments

in vitro

tests. Human gastric

cancer AGS and HGC-27 cells were treated with PKM2 and C-Myc lentivirus, the effects of knockdown PKM2 or C-MYC were

analyzed on cell proliferation, cell apoptosis, the ability of cell migration and growth signaling pathway

in vitro

. The expression of

PKM2, C-MYC, LDHA, STAT3, P-STAT3, GLUT-1 gene was identified by quantitative real-time polymerase chain reaction and

western blotting, Lactate and glucose levels were tested by the corresponding kit. Our findings showed that PKM2 and C-MYC were

up-regulated in human gastric cancer. Knockdown C-MYC in gastric cancer cells suppressed cell proliferation capacity and glycolysis

level, co-knockdown of PKM2 and C-MYC, the inhibitory effect on gastric cancer cells was more obvious compared to knockout

PKM2 or C-MYC alone. And there was a correlation between mTOR/PKM2 and STAT3/C-myc signaling pathways. Our results

suggest that C-MYC may be considered a potential therapeutic target for gastric cancer, and PKM2 combined with C-MYC can better

inhibit the malignant biological behavior of gastric cancer.

Biography

Chen Min has completed her PhD from Wuhan University, School of Medicine. She is an associated Physician working in the affiliated Drum Tower Hospital of

Nanjing University, Medical School, P R China for almost seven years. She is In Charge of teaching work in the Department as Teaching Secretary. She has

published more than 10 papers in reputed journals. Her research field is about basic and clinical research in gastric cancer.

croweminchan@gmail.com

Chen Min et al., J Gastrointest Dig Syst 2018, Volume 8

DOI: 10.4172/2161-069X-C1-064