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conferenceseries
.com
Volume 8
Journal of Gastrointestinal & Digestive System
ISSN: 2161-069X
Bariatric Surgery 2018 & Gastro 2018
March 15-16, 2018
JOINT EVENT
12
th
Global Gastroenterologists Meeting
3
rd
International Conference on Metabolic and Bariatric Surgery
&
March 15-16, 2018 Barcelona, Spain
CrosstalkofmTOR/HIF-1a/PKM2 andSTAT3/C-MYCsignalingpathways regulate the energymetabolism
and acidic microenvironment of gastric cancer
Chen Min, Sumeng Gao
and
Xiaoping Zou
The Affiliated Drum Tower Hospital - Nanjing University Medical School, P R China
C
ancer cells consume large amounts of glucose to produce lactate, even in the presence of ample oxygen. This phenomenon is
called the Warburg effect. C-Myc is an important member of the Myc gene family and is involved in the development of various
tumors. It plays an important role in the regulation of tumor energy metabolism which can regulate glycolysis to promote tumor
Warburg effect. Our study aims to improve the malignant biological behavior by controlling the energy metabolism of gastric cancer
through the mTOR/HIF-1α/PKM2 and STAT3/c-Myc signaling pathway through a series experiments
in vitro
tests. Human gastric
cancer AGS and HGC-27 cells were treated with PKM2 and C-Myc lentivirus, the effects of knockdown PKM2 or C-MYC were
analyzed on cell proliferation, cell apoptosis, the ability of cell migration and growth signaling pathway
in vitro
. The expression of
PKM2, C-MYC, LDHA, STAT3, P-STAT3, GLUT-1 gene was identified by quantitative real-time polymerase chain reaction and
western blotting, Lactate and glucose levels were tested by the corresponding kit. Our findings showed that PKM2 and C-MYC were
up-regulated in human gastric cancer. Knockdown C-MYC in gastric cancer cells suppressed cell proliferation capacity and glycolysis
level, co-knockdown of PKM2 and C-MYC, the inhibitory effect on gastric cancer cells was more obvious compared to knockout
PKM2 or C-MYC alone. And there was a correlation between mTOR/PKM2 and STAT3/C-myc signaling pathways. Our results
suggest that C-MYC may be considered a potential therapeutic target for gastric cancer, and PKM2 combined with C-MYC can better
inhibit the malignant biological behavior of gastric cancer.
Biography
Chen Min has completed her PhD from Wuhan University, School of Medicine. She is an associated Physician working in the affiliated Drum Tower Hospital of
Nanjing University, Medical School, P R China for almost seven years. She is In Charge of teaching work in the Department as Teaching Secretary. She has
published more than 10 papers in reputed journals. Her research field is about basic and clinical research in gastric cancer.
croweminchan@gmail.comChen Min et al., J Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C1-064