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Volume 7, Issue 1 (Suppl) J Ecosyst Ecogr
ISSN: 2157-7625 JEE, an open access journal Biodiversity Congress 2017
April 27-28, 2017
April 27-28, 2017 Dubai, UAE
6th International Conference on
Biodiversity and Conservation
Isolation, identification, and characterization of rubber degradation by a mixed microbial culture
Munzer Ullah and Zhu Hu
China University of Petroleum China
The present research was directed to exploit the potential bacterial and fungi strains for the biodegradation of Polyisoprene
(Natural Rubber). In current study, twelve different bacterial strains were isolated from the sewage sludge sample through
enrichment and soil burial techniques. The microbes isolated from the samples showed good growth and biodegradation activities
on culture medium. Later on these strains were identified and characterized on the basis of morphological and biochemical test.
Among these, seven were Bacillus, three Aerococus and two were Staphylococcus sp. Consequently, two fungal strains were also
identified and named as Aspergillus sydowii and Aspergillus candidus. Biodegradation activates of the mixed culture of microrganism
were monitored through UV spectrophotometer and functional groups changes were analyzed through FTIR spectroscopy. Fourier
Transform Infrared Spectroscopy (FTIR) (Bio-Rad Merlin) analysis displayed the changes in the region 1200-1400 cm-1 representing
the degradation of Polyisoprene rubber after culturing in liquid media. CO2 evolution in Sturm test was gravimetrically calculated as
42.88 g/l in case of test. However 20.58 g/l in case of control was noted. The difference in amount of CO2 produced both in test and
control indicated the degradation of rubber. This study determined that a mixed microbial culture had a potential tendency toward
the degradation of rubber.
Biography
Munzer Ullah did Master and MPhil. from Quaid –e-Azam university Islamabad Pakistan and currently enrolled in prestigeous Center of Bioengineering and
Biotechnology Chinas University of Petroleum. East China. Zhu hu is currently working as a prof in the said center.
Munzer Ullah et al., J Ecosyst Ecogr 2017, 7:1(Suppl)