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.com
Volume 8, Issue 5 (Suppl)
J Bioremediat Biodegrad, an open access journal
ISSN: 2155-6199
Biofuels Congress 2017
September 05-06, 2017
September 05-06, 2017 | London, UK
Biofuels and Bioenergy
6
th
World Congress on
Henry T Bedoya et al., J Bioremediat Biodegrad 2017, 8:5(Suppl)
DOI: 10.4172/2155-6199-C1-008
Production of microalgae biomass: Upscaling of five commercial microalgae strains in greenhouse at
northern latitudes
Henry T Bedoya
1
, Kumar Sahu
2
, Hans R Gislerød
1
and
Bjørn Rusten
2
1
Norwegian University of Life Sciences, Norway
2
Aquateam COWI AS, Norway
Statement of the Problem
: The feasibility of upscaling of microalgae biomass at Norwegian latitudes in greenhouse without
artificial illumination nor extra heating as a future sustainable energy source has been evaluated.
Methodology & Theoretical Orientation
: Two marine(m) and three freshwater(f) algae were cultivated in duplicate, from
inoculum batch to upscale in polypropylene open Tray PhotoBioReactor (TPBR, 25 L).
Chlorella vulgaris(f), Dunaliella
salina(m), Nannochloropsis oculata(m)
, cultivated for 63 days (20.06.12-23.08.12), and
Scenedesmus sp(f), Chlorella wild mix
Årungen(f),
cultivated for 42 days (20.07.12-23.08.12), at semi-continuous operation system, enriched CO
2
air (3%) and
prepared in situ, trifold nitrogen nutrition media (3N-BBM+Vit) and tap water, with volumes replenished when need. Effects
investigated: 1. Irradiance and temperature on specific growth rate and daily growth. At 23°C
Scenedesmus
sp. grew faster
at 0.05 h-1 and fivefold when doubling the irradiation energy input, meanwhile
Dunaliella salina
, reported 0.024 h
-1
and
71.4% growth increase. 2. Outside weather condition in conjugation with irradiation and temperature on oxygen evolution
(dissolved, DO) showed that cloudy days generated 31% more DO with 2.64 times less PAR irradiation than sunny days. 3.
Optimized Dilution (D) and Mixing (M) regimes on biomass productivity (P) of marine algae increased by 60%. 4. Irradiance
(I) on Photosynthetic Efficiency (PE), for marine strains 61% lower irradiance gave 4 times higher PE, and for freshwater
strains, a four times lower irradiance gave 4.6 times higher PE. 5. Irradiance on areal CO
2
fixation rate, the mean CO
2
fixation
rate was 55.44 gCO
2
m
-2
d
-1
, which is 2.6 times higher values than found by Hulatt (2011). 6. Outdoors weather conditions on
TPBRs energetic efficiency found the overall Irradiation Utilization Efficiency (IUE) provided by the TPBR.
Nannochloropsis
oculata
, performed best with 1.37 gMJ-1, and optical pathway 0.09 m.
Conclusion & Significance
: A cost-efficient greenhouse microalgae biomass production at northern latitudes shows great
potential as sustainable energy supplier e.g., raw ingredients for nutraceutical and animal feed industries.
henry.brujillo@gmail.com