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Volume 9, Issue 5 (Suppl) J Cancer Sci Ther, an open access journal
ISSN: 1948-5956 Cancer Stem Cells and Oncology Research 2017
June 26-28, 2017
Page 41
10th International Conference on
June 26-28, 2017 London, UK
CANCER STEM CELLS AND
ONCOLOGY RESEARCH
Stem/progenitorcells inhumanmilkand relations betweennumberof cells inhumanmilkandbreastfeeding
mother-infant dyad
Dzwigala M E1, 2, Zychowicz M2, Sarnowska A2 and Romejko-Wolniewicz E1
1Medical University of Warsaw, Poland
2Mossakowski Medical Research Centre- Polish Academy of Sciences, Poland
Human breast milk consists of different types of cells: leukocytes, epithelial cells, fibroblasts and pericytes. The aim of this study
was to confirm presence of stem/progenitor cells in human milk, to evaluate their pluripotent and regenerative potential and
to test correlation between mother, infant and number of cells in human milk. Fresh milk samples were acquired from women 0-7
days post-delivery. The consent according to the Ethics Committee of Warsaw Medical University guideline was obtained from each
woman. The samples were collected manually or by breast pump from 47 mothers. Cells isolation was performed within 4 hours
after sample collection. Various types of media were used in cells culture (MammoCult, DMEM + 10% FBS and others). Cells were
characterized by flow cytometry (FC), RT-PCR and immunocytochemistry. Health status of the mother and the child was estimated.
Anthropometric data was obtained from patients’ history. Stem/progenitor cells present in human milk displayed heterogeneous
expression of pluripotency genes characteristic for human embryonic stem cells such as: transcription factors OCT4, SOX2 and
NANOG. No statistical relationship was found between number of cells in human milk and any of the following: previous surgical
procedures, marital status, smoking during pregnancy, regular or irregular menstruation cycle, child’s sex and others. Negative
correlation (r=-0.5384, p<0.0012) was found between the day of sample collection and the number of milk cells. The study confirms
presence of stem/progenitor cells in human breast milk and the correlations might argue the decreasing number of cells in human
breast milk during first week from delivery.
Biography
Dzwigala M E is a 5th year student of Faculty of Medicine at the Warsaw Medical University, Poland. In 2011, she received her Master of Science degree from
the Warsaw School of Economics. From 2015, she started taking part in projects carried out by Students' Scientific Group in the Department of Obstetrics and
Gynecology under the supervision of Assoc. prof. Ewa Romejko-Wolniewicz (Chief of Department Krzysztof Czajkowski PhD, MD) and she is also involved in
basic research working in the Translative Platform for Regenerative Medicine, Medical Research Centre, Polish Academy of Sciences under supervision of Anna
Sarnowska PhD, MD (Chief of the Platform of Regenerative Medicine in Polish Academy of Sciences). She is interested in the Stem Cell Biology field, especially
human mesenchymal stem cells, human placenta stem cells, human breast stem cells and cervical cancer stem cells. In the area of obstetrics and gynecology, she
is involved in the research concerning pregnancy programing, intrauterine infection, amniotic fluid microbiome, pregnancy diabetes and hypertension, perinatology.
Dzwigala M E et al., J Cancer Sci Ther 2017, 9:5(Suppl)
DOI: 10.4172/1948-5956-C1-102