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conferenceseries.com
October 24-25, 2016 | Valencia, Spain
International Conference on
Environmental Health & Safety
Volume 4, Issue 5 (Suppl) Occup Med Health
ISSN:2329-6879 OMHA, an open access journal Environmental Health 2016
October 24-25, 2016
MOLECULARHIGH-RESOLUTIONMONITORINGOFLISTERIAMONOCYTOGENESONFOOD
PRODUCTS AND FOOD-ASSOCIATED ENVIRONMENTS
Olivier Joussona
aCentre for Integrative Biology (CIBIO), University of Trento, Italy
We present the latest insights concerning molecular methods for qualitative and quantitative foodborne pathogen detection and
characterization, with a focus on Listeria monocytogenes, a facultative intracellular human pathogen. The high mortality rate
of listeriosis (around 20%) and the persistence of Listeria monocytogenes in food-associated environments makes its elimination or
reduction a compulsory step before marketing potentially contaminated products. Besides methods based on cultural, immunological,
or biochemical identification and enumeration of Listeriamonocytogenes, a number of molecular methods with strain-level resolution
have been developed in recent years for epidemiological investigations. These methods mainly include real-time quantitative PCR
(qPCR), pulsed-field gel electrophoresis (PFGE), multi-locus variable number tandem repeat analysis (MLVA), multi-locus sequence
typing (MLST), multilocus genotyping (MLGT) and whole genome sequencing (WGS). Subtyping of L. monocytogenes strains can
prove to be crucial to point source outbreaks in food processing plants. The emergence of WGS as a routine method should assist in
the development of prospective epidemiological surveillance programs for L. monocytogenes and other pathogens relevant to public
health.
Biography
Olivier Jousson has completed his PhD at the age of 28 years from the University of Geneva (Switzerland) and postdoctoral studies from University Hospital
Lausanne. He is Associate Professor and PI of the Microbial Genomics Laboratory at CIBIO. He is director of teaching of undergraduate biotechnology programs
at the University of Trento. He has published more than 60 papers in international, peer-reviewed journals. He is interested in the study of bacterial pathogens,
including genome-wide identification and functional characterization of virulence factors, development of molecular typing systems, and population biology and
epidemiology. He has been serving as a reviewer or invited editor for a number of reputed journals in the field.
Olivier Jousson, Occup Med Health Aff 2016, 4:5 (Suppl)