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conferenceseries
.com
Volume 7, Issue 2 (Suppl)
Clin Exp Pharmaco
ISSN: 2161-1459 CPECR, an open access journal
Ethnopharmacology 2017
March 23-25, 2017
March 23-25, 2017 Orlando, USA
5
th
International Conference and Exhibition on
Pharmacology and Ethnopharmacology
Identification and quantification of polyphenols in
Sorghum bicolor (L) Moench
leaves extract using
reverse-phase HPLC-DAD
F B Borokini
Rufus Giwa Polytechnic, Nigeria
P
lants are used traditionally, as medicine due to their antioxidant activity which is due to their ability to form secondary metabolites
which are used to restore health and play important role in treatment of many diseases. The use of the plants, plant extracts and
isolated bioactive compounds from natural sources has been a lead that provided the foundation to modern medicine. In Nigeria,
extracts from the leaves of S. bicolor is commonly used in traditional medicine for treatment of ailments and as blood supplement.
The phytochemicals screening of the aqueous, methanol and ethanol extracts of the
Sorghum bicolor
showed the presence of some
bioactive compounds. The aqueous extract indicated flavonoids and terpenoids and absence of alkaloid, saponin, tannin, steroid,
anthraquinone and phlobatannin. However, saponin, tannin, flavonoid and terpenoid were found in the methanol extract while
alkaloid, steroid, anthraquinone and phlobatanin were absent. The flavonoids and phenolic acids contained in the extract were
investigated using Reverse-Phase HPLC-DAD and the concentrations of the identified phenolic compounds in mg/g were: Gallic
acid (13.54 ±0.02), catechin (9.29±0.01), chlorogenic acid (13.48±0.01), caffeic acid (52.17±0.03), ellagic acid (32.64±0.02), rutin
(41.28±0.01), isoquercitrin (16.71±0.02), quercitrin (83.52±0.03) quercetin (70.49±0.01) and kaempferol (71.16±0.01). The result in
this study shows that the extract is rich in polyphenols with antioxidant potential and may validate the traditional medicinal use of
the S. bicolor leaves extract.
Biography
F B Borokini is a Chemistry Lecturer and Researcher with over 16 years of postgraduate teaching and research experience in both secondary and tertiary
institutions in Nigeria. She currently teaches Chemistry in the Science Laboratory Technology Department, Rufus Giwa Polytechnic, Owo, Ondo State, Nigeria. She
had her Master of Technology in Analytical Chemistry and PhD in Food Chemistry from the Federal University of Technology, Akure, Ondo State, Nigeria. She has
served as the Departmental Course Adviser and the Faculty of Applied Science Representative in her institution. She is very resourceful and has some scientific
publications in reputable journals.
borokinif59@yahoo.comF B Borokini, Clin Exp Pharmacol 2017, 7:2 (Suppl)
http://dx.doi.org/10.4172/2161-1459.C1.016