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conferenceseries
.com
Volume 8, Issue 3 (Suppl)
J Clin Cell Immunol, an open access journal
ISSN: 2155-9899
Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8
th
European
Immunology Conference
In
silico
study of lipids associated in non-bilayer phospholipid arrangements
Sánchez B Sandra
1
, Landa S Carla E
1
, Reséndiz M Claudia A
1
, Wong B Carlos
1
, Wong R Carlos
1
, Yáñez M Cornelio
2
, Castelán V Juan Arturo
3
, Domínguez D
Laura
3
and
Baeza R Ma Isabel
1
1
ENCB- The National Polytechnic Institute, Mexico
2
Computer Research Center-IPN, Mexico
3
National Autonomous University of Mexico, Mexico
Statement of the Problem:
Systemic lupus erythematous (SLE) is an autoimmune, chronic and multifactorial disease. For SLE study,
some animal models had been developed, and our lab has one. Our model was obtained by administering a different lipid bilayer
structure called non-bilayer phospholipid arrangements. These non-bilayer phospholipid arrangements can be stabilized with drugs
such as chlorpromazine or procainamide and specifically in female mice, causing a disease very similar to human SLE. To help
understand how these lipid structures in the development of the disease involved, we used bioinformatics tools to understand how
they form, and now we’ll try to extrapolate this to explain some of the symptoms faced by patients with SLE.
Methodology &Theoretical Orientation:
A molecular simulation was established using all the conditions used in our murine model
with chlorpromazine and was created with GROMACS software. Analyses of results were made with GROMACS utilities, proprietary
Python programs and VMD tools.
Findings:
With our simulation strategy, we were able to observe differences between diverse lipidic environments with one or various
molecules of chlorpromazine. Also, we have started new simulations to improve our strategy and get more useful information for our
molecular knowledge of the models and the etiology of the human disease.
Conclusion & Significance:
These findings help us to understand how the models are triggered and give us clues about how to
improve them for a further research of how SLE is initiated in humans. That probably will contribute to the improvement of the
models and support our novel theory about the etiology of the SLE disease in humans.
Biography
Sánchez B Sandra has her expertise in Molecular Biology and Bioinformatics, and is passionate about Biochemistry and Science Communication. Her novel
approximation to the function of a drug on a bilayer membrane can help to better understand our murine models and how this can impact our knowledge on the
systemic lupus erythematosus disease. She has built her abilities after years of experience in research, project administration and protocols development in the
Pharmaceutical Industry, teaches of Biochemistry and Genetics for physicians, and translates pharmaceutical documents and participates in science fairs for kids.
This work was possible due to her willingness to collaborate with external researchers, as Laura Domínguez from FQ-UNAM and Cornelio Yañez from CIC-IPN,
who gave her the tools and knowledge to use bioinformatics and a Mexican Supercomputer to realize this project.
profra.sandrasb@gmail.comSánchez B Sandra et al., J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037