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conferenceseries
.com
Euro Pathology 2018 | Hematologic Oncology 2018
June 20-21, 2018
Volume 8
Journal of Clinical & Experimental Pathology
ISSN : 2161-0681
15
th
EUROPEAN PATHOLOGY CONGRESS
&
LEUKEMIA AND HEMATOLOGIC ONCOLOGY
June 20-21, 2018 | Paris, France
14
th
International Conference on
JOINT EVENT
Correlation of histomorphology and immunohistochemistry in histiocytic and dendritic cell neoplasms: A
retrospective observational study of three years in a referral laboratory in India
Shivani Sharma, Sambit Mohanty, Kunal Sharma, Aditi Dewan, Ekta Jain, Bhawna and Lata Kini
CORE Diagnostics, India
H
istiocytic and dendritic cell neoplasms (HDCN) are rare disorders of lymph node and soft tissue. These neoplasms include
histiocytic sarcoma (HS), langerhans cell histiocytosis (LCH), follicular dendritic cell sarcoma (FDCS), interdigitating cell
sarcoma (IDCS), indeterminate cell sarcoma (INDCS), and fibroblastic reticular cell tumor (FRCT). These neoplasms are often
misdiagnosed as carcinoma, neuroendocrine tumors and other Non-Hodgkin lymphomas. We sought to establish a correlation
between the histomorphologic features and immunohistochemical results of these neoplasms and analyse the diagnostic pitfalls.
This is a retrospective observational study including 17 cases of HDCN collected over a period of three years. Formalin fixed
paraffin embedded tissue underwent routine haematoxylin and eosin (H and E) staining and microscopic examination. An extensive
immunohistochemical panel was performed and included markers necessary for the diagnosis (CD68, NSE, CD1a, CD4, CD21,
CD23, cyclinD1, CD45, Ki67, vimentin, S100, and PanCK) and markers important to exclude other differentials. Among 17 cases,
LCH (8/17; 47%) was the most common subtype, and HS (7/17; 41.1%) was the second most common case, each of FDCS (1/17;
5.8%) and FRCT (1/17; 5.8%). The mean age of the patients was 36.6 years with male to female ratio of 2.4:1. LCH was seen in head
and neck region predominantly including occipital bone, scalp, subgaleal periosteum, external auditory canal, supraclavicular lymph
node, and cervical spine. The site of HS varied from colon (2/7), lymph node (1/7), lower limb (2/7) and spine (2/7). FDCS was
diagnosed in a lymph node and FRCT presented as an abdominal mass. For LCH, on morphology, grooved nuclei with longitudinal
cleft in the Langerhans cells seen in a background eosinophilic infiltrate were the most striking morphologic feature (8/8; 1.00%).
CD1a, S100 and CD68 co-positivity was seen in all eight cases of LCH. Histiocytic sarcoma showed a consistent positivity for
CD68 and CD4 in all the cases. FDCS showed characteristic histomorphologic features of tumor cells arranged in whorls, fascicles
and storiform pattern with positivity of CD21 and CD23. The tumor cells in FRCT were arranged in a storiform pattern and showed
positivity for vimentin and SMA. HDCNs are a rare group of hematologic disorders that have variable clinical presentations, rare
sites and different prognoses. A careful morphologic evaluation and an extensive immunohistochemical work up are mandatory for
their diagnoses. As a future standpoint, more cases need to be pooled, studied and evaluated for genetic studies to attain optimal
treatment protocols for this rare group of neoplasms.
Biography
Shivani Sharma has completed her graduation from Maulana Azad Medical College, New Delhi in 2009, and completed her Diploma in Clinical Pathology from
Indira Gandhi Medical College, Shimla, Himachal Pradesh in 2012. She studied Diploma in National Board from Medanta-The Medicity, Gurgaon, Haryana (2013-
2015), also, cleared the first part of FRCPath, Royal College of London in March 2015. She has been awarded the Shiv Ram Vidyawanti Kakkar Memorial Award,
Gold Medal in ENT in 2007, and Gold Medal for the Best Student in Diploma in Clinical Pathology, in 2012 by Himachal Pradesh University. She has published
more than 14 papers in various reputed journals.
shivani.sharma@corediagnostics.inShivani Sharma et al., J Clin Exp Pathol 2018, Volume 8
DOI: 10.4172/2161-0681-C1-045