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Journal of Food: Microbiology, Safety & Hygiene | ISSN: 2476-2059 | Volume 3
December 03-04, 2018 | Chicago, USA
Food Safety & Regulatory
International Conference on
Water Microbiology, Water Sustainability and Reuse Technologies
3
rd
International Conference on
&
Microbial community structure in an innovative membrane bioreactor treating sugar mills spent wash-water
Zubair Ahmed
Mehran University of Engineering and Technology, Pakistan
T
he sugar industry is the largest industry in Pakistan, contributing 3.2% of GDP in agriculture. It produces alcohol and byproducts
like molasses. However, it is also producing a significant amount of untreated wastewater, which is disposed of directly into canals
by the distilleries and sugar manufacturer industries which causes serious problem to water resources and pollutes the environment.
Only a few sugar industries are treating wastewater, but they have unsuitable treatment methods. Among wastewater and effluent
treatment methods anaerobic digestion with a membrane bioreactor (AnMBR) is a most suitable method of treatment, in which
waste is biologically broken down into biogas in the absence of oxygen, while filtration membrane helps to separate a tiny particle
from wastewater sludge. In the present study, a pilot-scale AnMBR assembly was inoculated with sludge, taken from an anaerobic
digester. The reactor was first accumulated using synthetic wastewater, followed by distillery wastewater. The pH of the reactor was
maintained between 7.2 to 8.5. Two samples from the initial stage (AnMBR) of synthetic wastewater and two samples after the
stabilizing the membrane bioreactor of distillery wastewater were collected. DNA extraction was carried out by using GeneJet DNA
purification kit. For 16S rDNA-based metagenomics for bacterial community characterization, a V3-V4 region of 16S rRNA gene
was amplified and amplicon libraries were sequenced by using Illumina MiSeq sequencer (Illumina Inc. USA). Quantitative insights
into microbial ecology 2 (Qiime2) was used for quality control analyses, quality filtration, taxonomic characterization, alpha, and beta
diversity analysis. Furthermore, fluorescence in situ hybridization (FISH) technique, comprising of eight bacterial oligonucleotide
probes (labeled at 5’ site with different fluorescent dyes, purchase from
biomer.net)will also be used to detect microbial cells of
different lineages. This study provides detailed insights into bacterial genera present in the sludge sample, responsible for favorable
wastewater treatment.
zahmed.uspcasw@faculty.muet.edu.pkJ Food Microbiol Saf Hyg 2018, Volume 3
DOI: 10.4172/2476-2059-C4-018