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Volume 2, Issue 4(Suppl)

J Exp Food Chem 2016

ISSN: 2472-0542 JEFC, an open access journal

Page 56

Notes:

Food Safety & Processing 2016

December 05-07, 2016

conferenceseries

.com

Food Safety, Processing & Technology

December 05-07, 2016 San Antonio, USA

10

th

Global Summit on

Enhanced destruction of

Salmonella enterica

in carrot and berry juices by a combination of

cinnamaldehyde and high pressure processing

Aura Daraba

1, 2

, Aubrey F Mendonca

1

, David Manu

1

, James S Dickson

1

, Joseph Sebranek

1

, Angela Shaw

1

and

Alan DiSpirito

1

1

Iowa State University, USA

2

University of Galati, Romania

Statement of the Problem

: In recent years several disease outbreaks were linked to unpasteurized juices contaminated with human

enteric pathogens such as

Salmonella enterica

and

Escherichia coli

O157:H7 (CDC 2011; EFSA 2015). While heat pasteurization and

canning can inactivate vegetative pathogens, such processes can destroy heat labile nutrients and negatively alter sensory characteristics

of juices. Also growing consumer demands for foods which are nutritious, fresh-like, and devoid of synthetic preservatives, have

forced juice manufacturers to explore non-thermal processes and natural antimicrobials for pathogen control in juices. The present

study investigated the effect of low concentrations of cinnamaldehyde combined with high pressure processing (HPP) for killing

S.

enterica

in carrot juice (CRJ) and a mixed berry juice (MBJ) at 4ºC.

Methodology &Theoretical Orientation

: CRJ (pH 6.25) and MBJ (pH 3.59) with added cinnamaldehyde (0.10, 0.15 and 0.25 μl/ml)

were inoculated with

S. enterica

(5-strain; final concentration ~107 CFU/ml). Inoculated juices without added cinnamaldehyde served

as control. Juices (4ºC) were packaged in polyester pouches and pressurized (400 or 300 MPa) for 60, 90 and 120 seconds. The time

between inoculation and HPP was approximately 1.5 hours.

Salmonella

survived for 42 days or more in control CRJ following HPP

(400 MPa) for 30, 60, or 120 s. Addition of cinnamaldehyde to juices increased the sensitivity of

S. enterica

to HPP. Cinnamaldehyde

(0.25 µl/ml) combined with 400 MPa (60 s) inactivated

S. enterica

by more than 5.5-log in CRJ. In MBJ, cinnamaldehyde (0.15 µl/ml)

with a lower pressure (300 MPa for 120 s) resulted in complete inactivation (negative enrichment) and greater than a 5-log10 CFU/

ml reduction of

S. enterica

.

Conclusion & Significance

: The use of CA in conjunction with HPP has good potential to serve as an alternative process for heat

pasteurization of juices and meet the 5-log reduction performance standard as stipulated in the juice HACCP regulations.

Biography

Aura Daraba has her expertise in Food Safety and Food Quality, use of natural antimicrobials to control pathogens, use of non-thermal food processing technologies, and

implementation of HACCP in Food Industry and in Food Service Units. She has worked extensively along with Dr. Aubrey Mendonca and Dr. Angela Shaw in the use of High

Pressure Processing and use of Natural Antimicrobials to control pathogens in foods.

aura.daraba@gmail.com

Aura Daraba et al., J Exp Food Chem 2016, 2:4(Suppl)

http://dx.doi.org/10.4172/2472-0542.C1.006