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Notes:
Volume 6, Issue 3 (Suppl)
J Hypertens, an open access journal
ISSN: 2167-1095
Page 73
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Hypertension 2017 & Nuclear Cardiology 2017
September 11-13, 2017
JOINT EVENTON
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September 11-13, 2017 | Amsterdam, Netherlands
2
nd
International Conference on
Hypertension & Healthcare
2
nd
International Conference on
Non-invasive Cardiac Imaging, Nuclear Cardiology & Echocardiography
Altered NMDAreceptor-nitric oxide (NO) signaling in the rostral ventrolateral medulla contribute to
cardiovascular response of acute ethanol-exposure
Hsuan Lo
Tzu University, Taiwan
C
onsumption of ethanol (EtOH) has many effects on physiological functions particular in the central nerve system and
cardiovascular system. Hypotension is one of the main features of acute excessive intake of ethanol (alcohol intoxication).
In addition, chronic ethanol consumption has been associated with cardiovascular diseases, including hypertension and
stroke. The precise mechanisms underlying ethanol regulation of cardiovascular function remains unclear. It is well known
that central N-Methyl-D-Aspartate (NMDA) receptors are implicated in the modulation of glutamatergic transmission and
important in regulating neuronal activity. Recent evidence also suggests that nitric oxide is a key neuromodulator within the
central nervous system and its production is associated with NMDA receptor activation. The rostral ventrolateral medulla
(RVLM) is known as a vasomotor center, which provides the basal sympathetic outflow and maintains blood pressure (BP).
This study was carried out to test the hypothesis that NMDA and NO signaling in the RVLM are involved in the regulation of
ethanol-induced changes in BP. The BP response was measured in urethane anesthetized SD rats weighing 280-350 g. EtOH
(3.2 g/kg) was applied by intraperitoneal injection (IP). The samples of glutamate and NO were collected by micro dialysis
every 15 minutes in the RVLM and then analyzed with HPLC-ECD and NOx analyzer, respectively. The results showed that
IP ethanol elicited a significant increase in the level of glutamate and NO in RVLM and a reduction in BP. Microinjection
of ketamine (an NMDA receptor antagonist) or L-NNA (an NOS inhibitor) into the RVLM, which was applied 5 min after
administration of EtOH, attenuated EtOH-induced depressor effects. In addition, microinjection of ketamine suppressed the
level of NO production. The results suggest that increases in NMDA receptors activation and enhancement of NO synthase
activity in the RVLM may play a major role in ethanol-evoked hypotension.
Biography
Hsuan Lo is a PhD scholar, studied in the Department of Pharmacology and Toxicology at Tzu Chi University. His lab is interested in neuropharmacology particularly
in alcohol effects on the central nervous system and cardiovascular function. His studies focus on the mechanism of acute ethanol intake action on the glutamate
signaling within the rostral ventral lateral medulla (RVLM). He has performed the whole animal model with practiced skill in RVLM, microinjection of drug and
femoral artery surgery for measuring blood pressure, and also have set up the HPLC system to detect the neurotransmitter content. On the other hand, his master's
thesis was of Amyloid-beta peptides on NMDA receptor activation in rat sympathetic preganglionic neurons using whole cell, patch clamp technique. All his training
has led him to know more about, how the CNS works to control cardiovascular function.
tiansi0113@gmail.comHsuan Lo, J Hypertens 2017, 6:3(Suppl)
DOI: 10.4172/2167-1095-C1-003