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Volume 5, Issue 2 (Suppl)
Transcriptomics, an open access journal
ISSN: 2329-8936
Molecular Biology 2017
August 31-September 01, 2017
2
nd
International Conference on
August 31-September 01, 2017 Philadelphia, USA
Molecular Biology, Nucleic Acids &
Molecular Medicine
Using single-stranded DNA for homology directed repair catalyzed by CRISPR/Cas9 activity
Eric B Kmiec
Helen F Graham Cancer Center and Research Institute, USA
T
his workshop session will feature many speakers who are actively working in the field of gene editing with a specific focus
on the use of single-stranded DNA templates in combination with gene editing tools such as CRISPR/Cas9 to repair
genetic mutations via homology directed repair. Speakers will describe the design and rationale for using varying types of
single-stranded DNA molecules, ranging from short single-stranded oligonucleotides to long strands generated by multiple
amplifications in vitro. The overarching objective of all these projects is to reverse a point mutation or to restore gene function
by homologous fragment insertion. Each speaker will also detail experimental case studies in which their method of choice
was either successful or unsuccessful in generating the predicted genetic outcome. What were the consequences of these failed
attempts? The use of single-stranded DNA functioning as patching or bridging the resected section of DNA created by the
double-stranded break directed by CRISPR/Cas9 to reduce allelic heterogeneity will also be discussed. The format of the
workshop will be interactive and a healthy dose of participation by attendees will be highly encouraged. We hope to achieve an
understanding of how to study homology directed repair in mammalian cells in the most effective way, both transformed and
primary. We hope to define what is real, reproducible and robust? And what is also non-reproducible artefactual or fictional?
so that gene editing can grow in a truly healthy fashion, driven by science and not by publicity.
eric.b.kmiec@christianacare.orgTranscriptomics 2017, 5:2 (Suppl)
DOI: 10.4172/2329-8936-C1-013