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conferenceseries.com
May 01-02, 2017 Toronto, Canada
2nd International Conference on
Restorative Dentistry and Prosthodontics
Volume 5, Issue 1 (Suppl) J Oral Hyg Health
ISSN: 2332-0702 JOHH, an open access journal Restorative Dentistry & Prosthodontics 2017
May 01-02, 2017
Comparison of the biocompatibility of proroot MTA, MTAPlus and Retro MTAusing anMTT assay study
Negin Kouchak Dezfuli, Ehsan Asnaashari and Zohreh Khalilak
Tehran Medical Branch - Islamic Azad University, Iran
The purpose of this in vitro study is to evaluate and compare the cytotoxicity of three commercially available root-ending materials,
MTA Plus, Pro root MTA and Retro MTA, at different storage times after mixing on human periodontal fibroblast using a MTT
assay method. Varied concentrations (3, 6, 12, 25, 50 mg/ml) of the fresh and set root-ending materials (ProRoot MTA, MTA Plus
and Retro MTA) were placed in adjacent flasks of human periodontal fibroblast in DMEM medium within 96-well plates. Cellular
viability was evaluated using a MTT assay after 24, 48, and 72 h of initial mixing. The results were analyzed with 1-way ANOVA. The
results showed that there was no significant difference between the biocompatibility of MTA Plus, and that of Pro root MTA or Retro
MTA (P>0.05). Furthermore, no significant difference was observed between different time intervals for each group (P>0.05). The
current in vitro study showed almost similar biocompatibility for ProRoot MTA and MTA Plus and Retro MTA.
Biography
Negin Kouchak Dezfuli received a Doctor of Dental Medicine degree from the Faculty of Dentistry at the Islamic Azad University of Tehran in 2014. She has been
a Teacher Assistant during her study in the Cosmetic & Restorative department. She has worked as a General Dentist in Dental Treatment department of Alborz
University of Medical Sciences, Tehran, Iran, for one year. She is currently training the Oral Health Technicians in Alborz University of Medical Sciences. She is also
the member of the Dental Research Center of this university.
Negin Kouchak Dezfuli et al., J Oral Hyg Health 2017, 5:1 (Suppl)