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Volume 7, Issue 6(Suppl) J Chromatogr Sep Tech
ISSN: 2157-7064 JCGST, an open access journal Separation Techniques 2016
September 26-28, 2016
Page 46
Notes:
conferenceseries.com
Separation Techniques
September 26-28, 2016 Valencia, Spain
2nd International Conference and Expo on
Kinetic separation with MS detection: A “Swiss Army Knife” in selection and characterization of drug leads
The slowing discovery of new chemical entities in small-molecule drug development calls for disruptive approaches in drug
discovery. Among such approaches is selection of drug leads from ultra-diverse (>109 different structures) combinatorial
libraries. However, such selections as well as kinetic characterization of selected molecules constitute ultimate analytical
challenges: They require ultra-efficient separation without immobilization, highly-selective detection without labeling, and
data deconvolution algorithms for extracting kinetic information. We are developing a novel conceptual platform “Kinetic
Separation with MS Detection” for addressing these challenges. Uniquely, a single method of kinetic separation can be used for
3 major applications: Selection of drug leads and affinity probes from ultra-diverse libraries, kinetic characterization of binding
of drug leads and affinity probes to their protein targets and the use of affinity probes in disease diagnostics. We dub this
multi-blade tool an Analytical Swiss Army Knife. The concept of kinetic separation has been proven for 2 modes of separation:
Capillary electrophoresis and size-exclusion chromatography. We are now developing the application of kinetic separation to
development of drug leads from libraries of DNA-encoded small molecules and affinity probes from random DNA libraries.
In this lecture, the fundamentals of kinetic separation will be explained and examples of its practical use for selection and
characterization of drug leads and affinity probes will be presented. Prospective of the utilization of kinetic separation in the
pharmaceutical industry and academic research will be discussed.
Biography
Sergey N Krylov has obtained his PhD from Moscow State University and was trained as PDF at the University of Alberta. He has been a Professor of Chemistry
at York University in Toronto since 2000. He has held Canada Research Chair Tier II for a maximum term and is currently York Research Chair in Bioanalytical
Chemistry. He is the Founder and Director of the Centre for Research on Biomolecular Interactions. He is recognized internationally for his pioneering work in the
fields of chemical cytometry, kinetic analysis of affinity interactions and selection and applications of oligonucleotide aptamers. He has authored over 160 peer-
reviewed papers and his research contributions have been recognized with a number of distinctions including two awards from the Chemical Society of Canada.
Sergey N Krylov
York University, Canada
Sergey N Krylov, J Chromatogr Sep Tech 2016, 7:6(Suppl)