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Volume 3, Issue 1 (Suppl) Toxicol Open Access
ISSN: 2476-2067 TYOA, an open access journal Toxicology Congress 2017
April 13-15, 2017
April 13-15, 2017 Dubai, UAE
8th World Congress on
Toxicology and Pharmacology
Multi-residue analysis (GC-ECD) of some organochlorine pesticides in commercial broiler meat
marketed in Shivamogga city, Karnataka state
Lokesha L V1, Jagadeesh S Sanganal2, Yogesh Gowda S2, Shekhar2, Shridhar N B2, Prakash N1, Prashant kumar Waghe1,
H D Narayanaswamy2 and Girish Kumar V3
1Veterinary College, Shivamogga, India
2Veterinary College, Bengaluru, India
3Karnataka Veterinary, Animal & Fisheries Sciences University, India
Organochlorine (OC) insecticides are among the most important organotoxins and make a large group of pesticides.
Physicochemical properties of these toxins, especially their lipophilicity, facilitate the absorption and storage of these toxins
in the meat thus possessing public health threat to humans. The presence of these toxins in broiler meat can be a quantitative
and qualitative index for the presence of these toxins in animal bodies, which is attributed to waste water of irrigation after
spraying the crops, contaminated animal feeds with pesticides, and polluted air are the potential sources of residues in animal
products. Fifty (50) broiler meat samples were collected from different retail outlets of Bengaluru city, Karnataka state, in ice
cold conditions and later stored under -20oC until analysis. All the samples were subjected to Gas Chromatograph attached
to Electron Capture Detector (GC-ECD, VARIAN make) screening and quantification of OC pesticides viz; Alachlor, Aldrin,
Alpha-BHC, Beta-BHC, Dieldrin, Delta-BHC, o,p-DDE, p,p-DDE, o,p-DDD, p,p-DDD, o,p-DDT, p,p-DDT, Endosulfan-I,
Endosulfan-II, Endosulfan Sulphate and Lindane (all the standards were procured fromMerck). Extraction was undertaken by
blending 50 g of meat sample with 50 g sodium sulphate anhydrous, 120 ml of n-hexane, 120 ml acetone for 15 mins, extract
was washed with distilled water and sample moisture is dried by sodium sulphate anahydrous, partitioning was done with 25
ml petroleum ether, 10 ml acetonitrile and 15 ml n-hexane shaken vigorously for two minutes; sample cleanup was done with
florisil column. The reconstituted samples (using n-hexane) (Merck chem) were injected to Gas Chromatograph–Electron
Capture Detector (GC-ECD). The present study reveals that, among the 50 chicken samples subjected for analysis, 60% (15/50),
32% (8/50), 28% (7/50), 20% (5/50) and 16% (4/50) of samples were contaminated with DDTs, Delta-BHC, Dieldrin, Aldrin
and Alachlor, respectively. DDT metabolites, Delta-BHC were the most frequently detected OC pesticides. The detected levels
of the pesticides were below the levels of MRL (according to Export Council of India notification for fresh poultry meat).
Biography
Lokesha L V has completed his MVSc from Karnataka Veterinary Animal & Fisheries Sciences University, Bidar, Karnataka, India. He worked on Plant Toxicology,
“Toxicity studies of Ficus amplissima in rabbits & rats”. He has joined Karnataka Veterinary Animal & Fisheries Sciences University as Assistant Professor, in the
year 2011. Presently, he is pursuing his PhD degree programme from Veterinary College, Bengaluru and working on Residue Toxicology. He has been associated
with organizing two International Workshops on Comprehensive Toxicology-2015 and International Seminar on Leachables, Extractables & Residual Solvents, as
Treasurer. Further, he handled two university funded research projects, published 6 research papers in both national and international journals. He was awarded
with various awards, bagged first place for oral presentation in National Seminar on Ethnopharmacology, Department of Pharmacology and Toxicology, College
of Veterinary and Animal Sciences, Kerala and also bagged best poster award at 36th Annual Conference of Society of Toxicology (India) 2016 , held at Amity
University, Utter Pradesh.
Lokesha L V et al., Toxicol Open Access 2017, 3:1 (Suppl)