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.com
Volume 8, Issue 4 (Suppl)
J Vet Sci Technol, an open access journal
ISSN: 2157-7579
Veterinary 2017
September 04-05, 2017
September 04-05, 2017 | Paris, France
7
th
International Veterinary Congress
Listeria monocytogenes
in retailed raw chicken meat in Turkey
Belgin Siriken
1
, Naim Deniz Ayaz
2
and
Irfan Erol
3
1
Ondokuz Mayis University, Turkey
2
Kırıkkale University, Turkey
3
Ministry of Food Agriculture and Livestock, Turkey
Statement of the Problem:
The objective of this study to find the prevalence and antimicrobial resistance of
L. monocytogenes
from
a total of 116 chicken meat samples including 50 carcasses and 66 meat parts marketed in Turkey.
Methodology & Theoretical Orientation:
In the study, immunomagnetic separation (IMS) based cultivation technique, to detect
the hlyA gene for the verification of the isolates by PCR, and to identify the genoserotypes of the
L. monocytogenes
isolates by
multiplex PCR assay.
L. monocytogenes
isolates were also tested for their susceptibility to eight antibiotic (gentamicin, vancomycin,
chloramphenicol, streptomycin, tetracycline, ampicillin, penicillin G and erythromycin) agents using the disk diffusion method.
Findings:
51
L. monocytogenes
colonies were isolated from 34 (29.3%) chicken meat samples (11 [22.0%] carcasses and 23 [34.8%]
pieces of meat) by IMS based cultivation technique and confirmed by PCR. According to the multiplex PCR results, all the 51 isolates
were identified as genoserotype IIa (1/2a or 3a), 14 isolates (27.45%) were susceptible to all eight antimicrobial drugs tested, and the
remaining 37 isolates (72.54%) were resistant to gentamicin (one isolate 1.96), vancomycin (four isolates, 7.84%), penicillin G (six
isolates, 1.76%), streptomycin (nine isolates, 17.64%; resistant or intermediate), tetracycline (seven isolates, 13.72%) and ampicillin
(six isolates, 11.76%).
Conclusion & Significance:
In conclusion, a relatively high prevalence of
L. monocytogenes
was noted and the isolates were
characterized sporadic but sometimes epidemic serotypes. IIa in contrast to the most important epidemiological serotypes I. This
study results also showed that antimicrobial resistance is not frequent in the isolates. However, we did not detect number of
L.
monocytogenes
in analyzed samples. The presence of
L. monocytogenes
in chicken meat is concerning public health risk.
Biography
Belgin Sırıken is an expert in Food Microbiology, Safety and Chemical Properties of Particularly Animal Origin Foods. She has completed her PhD at Ankara
University, and now she is working as Prof. Dr. at Ondokuz Mayis University, Samsun, Turkey. Her focus is on Molecular Food Microbiology.
bsiriken@omu.edu.tr bsiriken@yahoo.comBelgin Siriken et al., J Vet Sci Technol 2017, 8:4(Suppl)
DOI: 10.4172/2157-7579-C1-024