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Volume 7, Issue 4 (Suppl) Int J Waste Resour
ISSN: 2252-5211 IJWR, an open access journal Waste Management Convention 2017
September 11-12, 2017
Page 30
Notes:
conferenceseries.com
September 11- 12, 2017 Singapore
5th World Convention on
Recycling and Waste Management
Screening of bacteria for bioplastic production
About 140 million tons of plastic are consumed every year worldwide, which necessitates the processing of approximately
150 million tons of fossil fuels and directly causes immense amounts of waste that can take thousands of years to naturally
deteriorate, if it degrades at all. Bacteria have the ability to produce bioplastics in the form of polyhydroxyalkanoates (PHAs)
and they are capable of producing PHA from various carbon sources ranging from inexpensive, complex waste effluents to
alkanes, fatty acids, plant oils and as well as simple carbohydrates. The major barrier to wide acceptance of bioplastic is the high
cost, particularly carbonaceous raw materials (40%) and polymer recovery (26%). Therefore, the aim of the present study is to
focusing on identification of alternative cost effective substrate for the production of PHA. Isolation of bacterial strains for the
screening of PHA producing ability carried out by using different oils as carbon source. The samples were collected from edible
and lubricating oil contaminated soil from garage and workshop. Isolation of bacterial strain was performed by serial dilution,
spread plate and enrichment technique. Screening was carried out by the Nile Blue A and Sudan Black B staining. The total
number of colony forming unit was 134×105 to 3×108 (Edible oil) and 75×105 to 26×108 (Lubricating oil). Out of 21 isolates
from edible oil and lubricating oil, 2 bacterial strain (E.O 1 and E.O 4) were fluoresce on plate with Nile blue A indicates the
positive result and show characteristic granule with Sudan Black B, indicating the presence of PHAs. Bacterial strains were
identified as Pseudomonas sp., (edible oil), -rods and Bacillus sp. (Lubricating oil), +rods. Production of PHA through these
strains will be carried out to get the sufficient amount of bioplastic.
Biography
Padma Singh is a Professor and Head of the Department of Microbiology, Girls Campus, Gurukul Kangri University, India. She has obtained her MSc degree (Gold
Medal) and PhD from Jiwaji University, Gwalior (MP). She has published more than 80 research papers and review articles in various national and international
journals. She has been honored with National APSI Award and Gold Medal in 2005-06 for organizing APSI national conference. She is a Member of various
academic professional bodies like AMI, IBS, ISCA, DUBS, APSI, and was awarded FAPSI and FBS. Her major research includes antimicrobial potential interest of
medicinal plant, biodeterioration, bioremediation and biodegradation.
Padma Singh
Gurukul Kangri University, India
Padma Singh, Int J Waste Resour 2017, 7:4 (Suppl)
DOI: 10.4172/2252-5211-C1-007