A Novel Nano-Capsule of ÃÂ±-Lipoic Acid as a Template of Core-Shell Structure Constructed by Self-Assembly
- *Corresponding Author:
- Hiromi Nishiura
Institute of Health Sciences
Ezaki Glico Co., Ltd., 4-6-5
Osaka 555-8502, Japan
E-mail: [email protected]
Received Date: October 23, 2012; Accepted Date: November 23, 2012; Published Date: November 27, 2012
Citation: Nishiura H, Sugimoto K, Akiyama K, Musashi M, Kubota Y, et al. (2013) A Novel Nano-Capsule of a-Lipoic Acid as a Template of Core-Shell Structure Constructed by Self-Assembly. J Nanomed Nanotechol 4:155. doi:10.4172/2157-7439.1000155
Copyright: © 2013 Nishiura H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Mast cells (MC) and peripheral blood basophils (PBB) are well known for their role in the allergic response mediated through high affinity IgE receptors (FcεRI). However, these cells can also be stimulated by other non-allergic secretagogues to release their inflammatory mediators. Certain fullerene derivatives (FD) have already been shown to stabilize FcεRI-mediated MC/PBB responses, but it is not know if they also stabilize these cells through non-IgEmediated mechanisms. A panel of FD was synthesized and tested for their ability to inhibit non-FcεRI mediated release from human MC and PBB. It was found that specifically engineered FD could significantly inhibit calcium ionophore, compound 48/80, somatostatin, and poly L-lysine induced MC degranulation and cytokine production, as well as blunt degranulation and cytokine production from N-formyl-methionine-leucine-phenylalanine (fMLP), poly L-lysine, and calcium ionophore stimulated PBB. The mechanism of inhibition was due in part to the prevention of secretagogueinduced increases in cellular reactive oxygen species (ROS) and calcium levels as well as the reduced activation of the MAPK signaling intermediates ERK1/ERK2 and LAT. Additionally, preincubation of MC with FD blunted the prostaglandin D2 (PGD2) production upon exposure to inflammatory stimuli. In both cell types, the extent of inhibition of mediator release in response to each secretagogue was dependent on the moieties/side chains attached to the carbon cage. These results further extend the utility of fullerene nanomaterials to control mediator release through non-IgE mediated pathways in MC/PBB.