A Study of Allergic Sensitization to Anisakis Species in Experimental Mice
Radwa G. Diab, Mona M. El Temsahy, Eman D. Elkerdany and Maha R. Gaafar*
Parasitology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt
- *Corresponding Author:
- Dr. Maha Gaafar
Faculty of Medicine, Alexandria University
E-mail: [email protected] com
Received date: September 25, 2010; Accepted date: March 25, 2011; Published date: April 05, 2011
Citation: Diab RG, El-Temsahy MM, Elkerdany ED, Gaafar MR (2011) A Study of Allergic Sensitization to Anisakis Species in Experimental Mice. J Bacteriol Parasitol 2:111. doi: 10.4172/2155-9597.1000111
Copyright: ©2011 Diab RG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Hypersensitivity to Anisakis species is a worldwide medical problem. The aim of this study was to detect the immunological response in experimental mice, through measurement of Immunoglobulin E (IgE) antibodies in their lymphocytes by flow cytometry, following the ingestion of Anisakis crude antigen.
Method: Sixty Swiss albino mice were divided equally into control and experimental groups and each of them were further subdivided into five subgroups, six mice each. The percentage of IgE antibodies was measured in the lymphocytes of their splenic suspensions at zero, 1st, 3rd, 5th and 7th weeks post inoculation using Fluorescein isothiocyanate (FITC) anti – mouse IgE and were analyzed on a FACS Calibur flow cytometer Becton Dickinson equipped with an argon-ion laser apparatus operating at 488 nm.
Results: The percentage of IgE antibodies was enhanced in lymphocytes of animals exposed to Anisakis antigen from the first week, peaking three weeks following initial exposure, starting a decline by week five and decreased more by the seventh week, as compared to the control group.
Conclusion: The results obtained from this study proved the efficacy of flow cytometry in detecting the sensitization against Anisakis species through the measurement of IgE antibodies.