alexa A Superficial Colon Tumor Model Involving Subcutaneous Colon Translocation and Orthotopic Transplantation of Green Fluorescent Protein-Expressing Human Colon Tumor | Abstract
ISSN: 2157-2518

Journal of Carcinogenesis & Mutagenesis
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

A Superficial Colon Tumor Model Involving Subcutaneous Colon Translocation and Orthotopic Transplantation of Green Fluorescent Protein-Expressing Human Colon Tumor

Heiying Jin1*, Zhijian Yang2, Jiangdong Wang3, Suying Zhang2, Yu Sun2 and Yijiang Ding1

1National center of colorectal surgery, the 3rd affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 1 Jinling Road, Nanjing 210001, P.R. China

2Origin Biosciences Inc., 5 Xinmofan Road, Nanjing 210009, P.R. China

3Department of Pathology, Nanjing general hospital of PLA, 305 zhongshandong road, Nanjing 210001, P.R. China

*Corresponding Author:
Heiying Jin
National Center of Colorectal Surgery
the 3rd Affiliated Hospital of Nanjing
University of Traditional Chinese Medicine. 1 Jinling Road
Nanjing 210001, P.R. China
Tel: 86-25-52276359
E-mail: [email protected]

Accepted date: September 28, 2010; Published date: September 28, 2010

Citation: Jin H, Yang Z, Wang J, Zhang S, Sun Y, et al. (2010) A Superficial Colon Tumor Model Involving Subcutaneous Colon Translocation and Orthotopic Transplantation of Green Fluorescent Protein-Expressing Human Colon Tumor. J Carcinogene Mutagene 1:104. doi: 10.4172/2157-2518.1000104

Copyright: © 2010 Jin H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: The orthotopic transplantation model of human tumor has been demonstrated to be more patientlike animal tumor model. However, observations of tumor progression and metastasis are limited by the deep location of the colon or limited deep penetration ability of fluorescence through tissue. The purpose of this study is to establish a superficial orthotopic model to allow easier real-time visualization and more sensitive monitoring of fluorescent orthotopic colon tumor. Methods: Human colon cancer HT-29 cells were transduced with a pLPCX expression retroviral vector containing GFP and neomycin resistance genes. For superficial orthotopic transplantation model, the cecum was identified and pulled out of the peritoneal cavity, the space between the cecum and peritoneum was sutured, the cecum was pulled to subcutaneous tissue, and an incision was made on the cecal serosa followed by the implantation of a 1-mm tumor tissue to the cecum. For comparison, a conventional orthotopic transplantation model was established in a separate group of mice simultaneously. When tumor sizes reached 5 mm in diameter, half the mice in each model received 5-FU treatment. Primary tumor and metastases were monitored by fluorescent imaging or caliber measurement. Results: Tumor fluorescence was observed as early as three days (median time of 4.7 ± 1.3 days) posttransplantation in the superficial orthotopic transplantation model, which was much earlier than 21 days (median time of 26.2 ± 9.9 days) in conventional orthotopic transplantation model. Although tumor growth of 5-FU treated mice in conventional orthotopic model were lower than those of the untreated mice, the difference was not significant. However, in superficial orthotopic model tumor growth was significantly inhibited in 5-FU treated mice relative to the untreated mice. Fluorescence imaging showed similar metastasis incidence between the superficial and conventional orthotopic transplantation models. Conclusions: The fluorescent superficial orthotopic transplantation colon model allows easier real-time visualization and more sensitive monitoring of tumor growth as well as convenient repeated sampling. It is a valuable orthotopic implantation model for evaluating colon cancer.

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version