alexa Antimicrobial, Antiviral Activity and GC-MS Analysis of
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential Oil Extracted from Achillea fragrantissima Plant Growing In Sinai Peninsula, Egypt

Gamil S G Zeedan1*, Abeer M. Abdalhamed1, Mahmoud E. Ottai2, Sobhy-Abdelshafy1 and Eman Abdeen3

1Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre,Dokki, Giza, Egypt

2Department of Genetics and cytology, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Giza, Egypt

3Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, University of Sadat City, Monifia, Egypt

*Corresponding Author:
Gamil S G Zeedan
Department of Parasitology and Animal Diseases
Veterinary Research Division, National Research Centre
P.O. Box 12622 El-Behouse Street, Dokki, Giza, Egypt
Tel.: +20233371499
Fax: +20233370931
Mobile: 01114513605
E-mail: [email protected]

Received date: September 20, 2014; Accepted date: October 07, 2014; Published date: October 14, 2014

Citation: Zeedan GSG, Abdalhamed AM, Ottai ME, Abdelshafy S, Abdeen E (2014) Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential Oil Extracted from Achillea fragrantissima Plant Growing In Sinai Peninsula, Egypt. J Microb Biochem Technol S8:006. doi: 10.4172/1948-5948.S8-006

Copyright: © 2014 Zeedan GSG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics in treatment of antibiotic-resistant bacteria. The present study aimed to evaluate the efficacy of essential oil extracted from Achillea fragrantissima plant growing in Egypt for antimicrobial, antiviral activities and chemical composition analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). We also performed determination of essential oil antimicrobial activity by agar desk diffusion method and Minimal Inhibitory Concentration (MIC). Also, antiviral activity on ORF virus by pock reduction test was performed. It was reduced virus titer from 5.9 to 1.00 at 180 minutes. Detection of beta lactams resistant bacteria (Gram-positive bacteria Staphylococcus aureus, Staphylococcus epidermidis (MRSA) and Gram-negative bacteria Escherichia coli) by PCR with primers of mecA gene and Bela gene. The essential oil obtained by hydrodistillation was analyzed by GC-MS. The major components were found to be Santolina triene (1.97%), 2,5,5-trimethyl-3,6-heptadien-2-ol (8.23%) Eucalyptol 8.17, trans-2,7-Dimethyl- 4,6octadien-2-ol (24.40%), 1,5-Heptadien-4-one-3,6-trimethyl (7.65%), Artemisia alcohol (3.49%), à Thujone (33.97%), Cissabinol (1.92%), Lavandulol (0.71%), 2-Octen-4-ol, 2-methyl (2.02%), 3-Cyclohexen1ol,4-methyl1 (1 methylethyl) (CAS) (2.15%), à terpineol ( 0.05% ), Estragole (0.71%) Lavandulyl acetate (0.49%), Sabinyl acetate (2.12 %), Germacrened (0.94%). Finally, our study proved that the essential oil has bactericidal effect on some bacterial resistant antibiotic (Gram-positive bacteria Staphylococcus aureus, Staphylococcus epidermidis (MRSA) and Gram-negative bacteria Escherichia coli ) as well as antiviral activity on ORF virus but it is still need further extensive studies for safety and drug interaction.

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