alexa Assembly of a Dual Aptamer Gold Nanoparticle Conjugate Ensemble in the Specific Detection of Thrombin when Coupled with Dynamic Light Scattering Spectroscopy
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

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Research Article

Assembly of a Dual Aptamer Gold Nanoparticle Conjugate Ensemble in the Specific Detection of Thrombin when Coupled with Dynamic Light Scattering Spectroscopy

Vishala S Ramyah Aaryasomayajula1, Tiffany Severs1, Kartik Ghosh2, Robert DeLong3, Xianfeng Zhang4, Saikat Talapatra4 and Adam K Wanekaya1*

1Chemistry Department, Missouri State University, Springfield, Missouri, USA

2Physics Department, Missouri State University, Springfield, Missouri, USA

3Biomedical Science Department, Missouri State University, Springfield, Missouri, USA

4Physics Department, Southern Illinois University, Carbondale, Illinois, USA

*Corresponding Author:
Adam K Wanekaya
Chemistry Department, Missouri State University
Springfield, Missouri, USA
Tel: +1-417-836-5611
Fax: +1-417-836-5507
E-mail: [email protected]

Received Date: June 03, 2014; Accepted Date: July 05, 2014; Published Date: July 10, 2014

Citation: Aaryasomayajula VSR, Severs T, Ghosh K, DeLong R, Zhang X, et al. (2014) Assembly of a Dual Aptamer Gold Nanoparticle Conjugate Ensemble in the Specific Detection of Thrombin when Coupled with Dynamic Light Scattering Spectroscopy. J Nanomed Nanotechnol 5:210. doi:10.4172/2157-7439.1000210

Copyright: © 2014 Aaryasomayajula VSR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

We demonstrate an extremely facile, rapid, specific and selective method for detecting proteins using aptamerconjugated gold nanoparticles coupled with dynamic light scattering (DLS) at ambient conditions. Addition of proteins to aptamer-conjugated gold nanoparticles (GNPs) induced the formation of protein-aptamer-gold nanoparticle conjugate complexes. The average hydrodynamic diameter of the nanoconjugate complexes as measured by DLS, increased with the corresponding increase in protein concentration. This correlation formed the analytical basis of the assay. The nanoparticles and nanoconjugate complexes were characterized by transmission electron microscopy, ultra-violet visible spectroscopy and DLS. Various parameters affecting the assay were optimized. Using thrombin as the model analyte, we demonstrated the detection of as low as 1.41 nM (0.05 μg/mL) of the protein. A linear dynamic range of up to 300 nM (11 μg/mL) was realized. The presence of other interfering proteins such as BSA showed no effect on the assay response.The presence of other interfering proteins such as bovine serum albumin (BSA) showed no significant effect on the assay response.

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