Characterization of Bone Marrow-Derived Dendritic Cells Developed in Serum-Free Media and their Ability to Prevent Type 1 Diabetes in Nonobese Diabetic Mice
Ben M Looney, Anna V Chernatynskaya, Michael J Clare-Salzler and Chang-Qing Xia*
Department of Pathology, Immunology and Laboratory Medicine, University of Florida, USA
- *Corresponding Author:
- Chang-Qing Xia
Department of Pathology, Immunology and Laboratory Medicine
University of Florida, USA, 1600 SW Archer Road
Gainesville, Florida 32610, USA
Fax: 0066 0 24113256
E-mail: [email protected]
Received date: January 20, 2014; Accepted date: March 15, 2014; Published date: March 22, 2014
Citation: Looney BM, Chernatynskaya AV, Clare-Salzler MJ, Chang-Qing Xia (2014) Characterization of Bone Marrow-Derived Dendritic Cells Developed in Serum-Free Media and their Ability to Prevent Type 1 Diabetes in Nonobese Diabetic Mice. J Blood Disord Transfus 5:206. doi:10.4172/2155-9864.1000206
Copyright: © 2014 Looney et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Dendritic cells (DC) have been investigated as a cell-based therapy for Type 1 Diabetes (T1D). BM-DC expanded ex vivo with GM-CSF and IL-4 is typically cultured with fetal bovine serum (FBS). The effect of FBS on NOD BM-DC has not been extensively studied. In the present study we compare BM-DC generated in serumfree culture media (X-VIVO20; FBS-) with BM-DC generated in media containing 10% FBS (RPMI1640/10%FBS; FBS+). We show that FBS- BM-DC display a phenotype and cytokine-producing profile distinct from FBS+ BMDC. Additionally, compared to FBS+ BM-DC, we show evidence of an altered Th cell response induced by FBSBM- DC. Finally, we demonstrate that only FBS- BM-DC prevent the onset of T1D and induce increased levels of CD4+Foxp3+ regulatory T cells as well as a long-lasting β cell-specific T cell response. This study indicates that serum-free media generates a more tolerogenic BM-DC capable of preventing T1D in the NOD mice.