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Characterization of Carbohydrate Surface Markers on Mouse Embryonic Stem Cells | OMICS International | Abstract
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

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Research Article

Characterization of Carbohydrate Surface Markers on Mouse Embryonic Stem Cells

Zhenwei He1,2, Yue An1,3, Gang Shi1,4, Yingwei Lin1,3, Jiliang Hu5* and Yali Li1*

1Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China

2Department of Neurology, Forth Affiliated Hospital of China Medical University, Shenyang, Liaoning Province China,

3Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116027, China

4Department of Colorectal Surgery, Liaoning Cancer Hospital and Institute, Cancer Hospital of China Medical University, Shenyang 110042, China

5Department of Neurosurgery, The Shenzhen People's Hospital (The Second Clinical Medical Collage of Jinan University), Guangdong 518020, China

Corresponding Authors:
Yali Li
Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
Tel: +86 411 8611 0221
E-mail:
[email protected]
Jiliang Hu
Department of Neurosurgery, The Shenzhen People's Hospital (The Second Clinical Medical Collage of Jinan University), Guangdong 518020, China
Tel:
+86 20 8522 0010
E-mail: [email protected]

Received Date: July 24, 2016; Accepted Date: August 12, 2016; Published Date: August 19, 2016

Citation: He Z, An Y, Shi G, Lin Y, Hu J, et al. (2016) Characterization of Carbohydrate Surface Markers on Mouse Embryonic Stem Cells. J Stem Cell Res Ther 6:353. doi: 10.4172/2157-7633.1000353

Copyright: © 2016 He Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Glycosylation of proteins and lipids on cell surface have been shown to be important in maintaining pluripotency and stem cell fate in embryonic stem cells. Lectins have been widely used to characterize carbohydrate modifications on cell surface of embryonic stem cells to determine pluripotency and stem cell fate. In the present study, a panel of 14 lectins and carbohydrate antibodies was used to characterize the carbohydrate surface markers of mouse Embryonic Stem (ES) Cells. SSEA-1-positive mouse ES cells were firstly enriched and the carbohydrate profile of the cells was determined by flow cytometry and immunocytochemistry. Enrichment of mouse ES cells yielded approximately 99.95 ± 0.87% of SSEA-1-positive mouse ES cells. A uniform and high percentage of binding was observed for PNA, DSL, JAC, GNL, PSA and LTL, with PNA, DSL, JAC and GNL having similar percentage of binding to SSEA-1 (99.9%), while PSA and LTL binding were approximately 95%-99%. Partial binding of WFL, SNA and AAL were observed in mouse ES cells which was also reflected by the respective immunocytochemistry images. A very low percentage of binding was observed for MAA and UEAI. The data showed that high expression of mannose, N-acetyllactosamine and galactose are present on the cell surface of mouse ES cells. Some reliable surface markers that can be used to determine pluripotency are PNA, DSL, JAC and GNL, which showed similar binding to SSEA-1, a well-established pluripotent marker. Taken together, the data has provided information on the cell surface carbohydrate profile of mouse ES cells.

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