Compatible Changes of Lead microRNAs in Circulating Plasma and Brain in Senescence-Accelerated Aging and Alzheimer's disease Mouse Models
Shephali Bhatnagar, Gregory J. Weber, Vikranth Shetty, Viktoriya Shekhovtsova and Eugenia Wang*
Advanced Genomic Technology, LLC, Louisville, Kentucky, 40202, USA
- *Corresponding Author:
- Eugenia Wang
5100 US Highway 42, Suite 433
Louisville, Kentucky, 40241
Tel: (502) 228-5438
E-mail: [email protected]
Received Date: June 23, 2014; Accepted Date: July 26, 2014; Published Date: August 04, 2014
Citation: Bhatnagar S, Weber GJ, Shetty V, Shekhovtsova V, Wang E (2014) Compatible Changes of Lead microRNAs in Circulating Plasma and Brain in Senescence-Accelerated Aging and Alzheimer’s disease Mouse Models. Aging Sci 2:125. doi: 10.4172/2329-8847.1000125
Copyright: © 2014 Bhatnagar S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Circulating microRNAs have recently emerged as powerful biomarkers because of their potential for monitoring changes in tissues such as the brain during aging. These microRNAs are found in many types of body fluids, and serve not only as systemic indicators of global and tissue-specific changes of gene expression, but also as functional mediators for cell-cell communication. In an effort to link changes in circulating microRNAs to changes in tissue, we used survival bleeding to perform comparative studies of three selected microRNAs, miR-34a, -34c, and -181b, in plasma and brain tissue in two types of animal models, Alzheimer’s Disease (AD) transgenic mutants and Senescence-Accelerated (SAMP8) mice. In this study, we show that expression of these microRNAs is altered in both AD and SAMP8 models, with comparable changes in expression of these specific microRNAs in both plasma and brain, and perhaps earlier in plasma. Our results demonstrate that the survival bleeding method allows longitudinal studies of changes in circulating microRNAs, which can be used as minimally invasive biomarkers of degenerative changes in mouse brain.