alexa Construction of Recombinant Escherichia coli JM109/LBH-10 and Comparison of Its Optimal Condition for Production of Carboxymethyl cellulase with Its Wild Type, Psychrobacter aquimaris LBH--10
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Construction of Recombinant Escherichia coli JM109/LBH-10 and Comparison of Its Optimal Condition for Production of Carboxymethyl cellulase with Its Wild Type, Psychrobacter aquimaris LBH--10

Sang-Un Lee1, Wa Gao2, Chung-Han Chung3 and Jin Woo Lee1,2*

1Department of Medical Bioscience, Graduate School of Donga-A University, Korea

2Department of Biotechnology, Dong-A University, Korea

3ReSEAT Programs, Korea Institute of Science and Technology Information, Korea

*Corresponding Author:
Jin Woo Lee
Department of Medical Bioscience, Graduate School of Donga-A University
Busan 604-714, Korea
Tel: +82-51-200- 7593
Fax: +82-51-200-7505
E-mail: [email protected]

Received date: February 25, 2014; Accepted date: March 18, 2014; Published date: March 21, 2014

Citation: Lee SU, Gao W, Chung CH, Lee JW (2014) Construction of Recombinant Escherichia coli JM109/LBH-10 and Comparison of Its Optimal Condition for Production of Carboxymethyl cellulase with Its Wild Type, Psychrobacter aquimaris LBH--10. J Microb Biochem Technol 6: 135-143. doi: 10.4172/1948-5948.1000134

Copyright: © 2014 Lee SU, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

A gene encoding the Carboxymethyl cellulase (CMCase) of a psychrophilic marine bacterium, Psychrobacter aquimaris LBH-10 was cloned in Escherichia coli JMB109. The optimal conditions of rice bran, ammonium chloride, and initial pH of the medium for cell growth of E. coli JM109/LBH-10 were 73.4 g/L, 5.8 g/L, and 6.5, respectively, whereas those for production of CMCase were 57.1 g/L, 6.4 g/L, and 6.7. The optimal temperatures for cell growth and the production of CMCase by E. coli JM109/LBH-10 were found to be and 40 and 35°C, respectively. The optimal agitation speeds and aeration rates of a 7 L bioreactor for cell growth and production of CMCase were 480 rpm and 1.0 vvm. In this study, the optimal conditions for cell growth and the production of CMCase by E. coli JM109/ LBH-10 were found to be different from those for its wild type, P. aquimaris LBH-10. The maximal production of CMCase by E. coli JM109/LBH-10 was 576.8 U/mL, which was 1.80 times higher than that by P. aquimaris LBH-10.

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