Diagnosis of Human BrucellosisSatish Gupte* and Tanveer Kaur
Department of Microbiology, Gian Sagar Medical College & Hospital, Rajpura, India
- *Corresponding Author:
- Satish Gupte
Gian Sagar Medical College & Hospital
E-mail: [email protected]
Received Date: October 17, 2015; Accepted Date: October 28, 2015; Published Date: November 04, 2015
Citation: Gupte S, Kaur T (2015) Diagnosis of Human Brucellosis. J Trop Dis 4:185. doi:10.4185/2329-891X.1000185
Copyright: © 2015 Gupte S, et al.This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Brucellosis is a worldwide zoonosis with a high degree of morbidity in humans. The disease may be overlooked and misdiagnosed because of the difficult diagnosis and the absence and lack of experience with laboratory testing. As the clinical picture of human brucellosis is fairly non-specific, a definitive diagnosis requires isolation of the causative organism, or the demonstration of the high levels of specific antibodies, or organism specific DNA in samples. Isolation of organism is time-consuming and hazardous, so must be performed by highly skilled personnel. So serological methods are preferred. Advanced serological methods like ELISA, Brucellacapt are more sensitive techniques. Combination of culture and serological test should be used to avoid misdiagnosis. Molecular assays come with high sensitivities and specificities and may reduce diagnostic delays in clinical laboratories. Several PCR based assays have been studied for standardizing them for brucellosis diagnosis. Another rapid, sensitive and inexpensive molecular technique, LAMP has been developed by designing primers specific for Brucella abortus genes. This technique can prove helpful in resource limited settings in developing countries. Highthroughput MLVA-16 genotyping techniques are also being studied and developed for brucella typing for tracing the source of brucellosis infection. This test can be useful for brucella diagnosis and surveillance studies. This review article describes procedures, advantages or limitations of some useful conventional diagnostic techniques to more sophisticated molecular techniques for brucellosis diagnosis.