alexa Estrogen-Dependent Downregulation of Hepcidin Synthesis
ISSN: 0974-8369

Biology and Medicine
Open Access

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Research Article

Estrogen-Dependent Downregulation of Hepcidin Synthesis Induces Intracellular Iron Efflux in Cancer Cells In Vitro

Jasmin Shafarin1, Khuloud Bajbouj1, Ahmed El-Serafy1,3, Divyasree Sandeep1 and Mawieh Hamad1,2*

1The Sharjah Institute for Medical Research, University of Sharjah, Sharjah, UAE

2Department of Medical Laboratory Sciences, University of Sharjah, Sharjah, UAE

3College of Medicine, University of Sharjah, Sharjah, UAE

*Corresponding Author:
Mawieh Hamad
Department of Medical Laboratory Sciences
College of Health Sciences, PO Box 27272
University of Sharjah, Sharjah, UAE
Tel: +9715057553
E-mail: [email protected]

Received Date: September 20, 2016; Accepted Date: October 31, 2016; Published Date: November 07, 2016

Citation: Shafarin J, Bajbouj K, El-Serafy A, Sandeep D, Hamad M (2016) Estrogen-dependent Downregulation of Hepcidin Synthesis Induces Intracellular Iron Efflux in Cancer Cells In Vitro. Biol Med (Aligarh) 8: 356. doi:10.4172/0974-8369.1000356

Copyright: © 2016 Shafarin J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

 

Abstract

It is well accepted that intracellular iron overload that associate with various forms of cancer fuels tumor mutagenesis and growth. Hence, iron chelation therapy is being increasingly used to minimize iron overload in cancer patients despite significant safety and efficacy concerns. Mounting evidence suggests that estrogen (E2) downregulates hepcidin synthesis and increases serum iron concentration. It is postulated therefore that, by downregulating hepcidin synthesis, E2 may maintain ferroportin integrity and enhance intracellular iron efflux. Here, MCF-7 and SKOV-3 cancer cells treated with increasing concentrations (5, 10 and 20 nM) of E2 were assessed for intracellular labile iron content, the expression of hepcidin, ferroportin, and transferrin receptors 1 and 2 along with cell viability at different time points post treatment. In MCF-7 cells, E2 treatment resulted in a significant reduction in hepcidin synthesis, most noticeably at the 20 nM/24 h dose, a significant increase in ferroportin expression and a marked decrease in transferrin receptors 1 and 2 expression. E2-treated cells also showed reduced intracellular labile iron content most evidently at 20 nM/48 h dose and reduced viability especially at 20 nM/72 h dose. E2-treated SKOV-3 showed slightly reduced intracellular labile iron content, reduced expression of hepcidin and significantly increased expression of TFR1 but not TFR2; FPN expression was overall similar to that of controls. The effects of E2 on intracellular iron metabolism in SKOV-3 were most evident at 5 nM/24 h dose. These findings suggest that E2 treatment induces intracellular iron efflux, which may minimize intracellular iron overload in cancer cells; disrupted expression of transferrin receptor 1 and/or 2 may help sustain a low intracellular iron environment.

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