Evaluation of Detached Leaf Assay for Assessing Leaf Rust (Puccinia triticina Eriks.) Resistance in Wheat
- *Corresponding Author:
- Woubit Dawit
Department of Plant Sciences and Horticulture
Ambo University, P.O. Box 19, Ambo, Ethiopia
E-mail: [email protected]
Received date: March 19, 2013; Accepted date: May 20, 2013; Published date: May 25, 2013
Citation: Boydom A, Dawit W, Getaneh W/Ab (2013) Evaluation of Detached Leaf Assay for Assessing Leaf Rust (Puccinia triticina Eriks.) Resistance in Wheat. J Plant Pathol Microb 4:176. doi:10.4172/2157-7471.1000176
Copyright: © 2013 Boydom A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The use of resistant cultivars is the most economical and effective method to control leaf rust of wheat caused by Puccinia triticina Eriks. Generally screening of wheat germplasm for resistance to rusts is carried out in the greenhouse and field conditions using seedling and adult plants. However, these screening trails are influenced by environmental factors and limit the number of races to be evaluated simultaneously. In the present study, a detached leaf assay was evaluated for screening of wheat germplasm resistance to leaf rust. As treatments, two senescence retardant chemicals (benzimidazole and kinetin) at different concentrations and combinations were added to 5% water-agar. The selected media was further validated in 20 wheat genotypes using three leaf rust races. The media containing 10 mg/L kinetin and 30 mg/L benzimidazole inoculated using an atomizer was the best in delaying the senescence and thereby increasing the sporulation. A positive correlation (r=0.9) between the detached leaf assay and the whole seedling assay infection types was observed. The standard errors for detached leaf and whole seedling assays were 0.24 and 0.3, respectively. The low standard errors further confirm the consistency of the disease response rating between the two assays. Generally this study revealed that the infection types were found to be similar both in detached leaf and whole seedlings assay. Therefore, detached leaf assay can be used to assess wheat genotypes against leaf rust.