alexa Further Molecular Characterization and Effect on Host Photosynthetic Pigments and Carbohydrate Pools of an Egyptian Isolate of TBSV
ISSN: 2157-7471

Journal of Plant Pathology & Microbiology
Open Access

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Research Article

Further Molecular Characterization and Effect on Host Photosynthetic Pigments and Carbohydrate Pools of an Egyptian Isolate of TBSV

Faiza A. Fattouh1, Awatif S. Ali2* and Radwa M. Fathy2

1Department of Botany and Microbiology, Faculty of Science, Alexandria University, Egypt

2Department of Botany, Faculty of Science, Damanhour University, Egypt

*Corresponding Author:
Awatif S. Ali
Department of Botany, Faculty of Science
Damanhour University, Egypt
Tel: +020-01281194206
E-mail: [email protected]

Received date: November 07, 2014; Accepted date: April 15, 2015; Published date: April 20, 2015

Citation: Fattouh FA, Ali AS, Fathy RM (2015) Further Molecular Characterization and Effect on Host Photosynthetic Pigments and Carbohydrate Pools of an Egyptian Isolate of TBSV. J Plant Pathol Microb 6:261. doi: 10.4172/2157-7471.1000261

Copyright: © 2015 Fattouh FA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



The Egyptian isolate of Tomato bushy stunt virus, the TBSV Egh was further characterized with respect to its coat protein gene (P42CP), movement protein gene (P22MP) and replication protein gene (P33RP). All 3 genes were amplified by PCR from TBSV Egh purified virus. Both P42CP and P22MP were further cloned, partially sequenced and deposited into the gene bank under accession numbers HM439101 NCBI and JX418297 respectively. Phylogenetic analysis illustrates evolutionary relationship between TBSV Egh P22 and P42 partial sequence indicated that this isolate may represent a new different strain of TBSV. Loss in chlorophyll content of TBSV Egh infected Lycopersicon esculentum and Cucurbita pepo hosts are reported. Variable significant differences in glucose, sucrose and polysaccharides have been observed


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