alexa Gene Silencing by siRNA Nanoparticles Synthesized via Sonochemical Method
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

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Research Article

Gene Silencing by siRNA Nanoparticles Synthesized via Sonochemical Method

Shimanovich U1*, Munder A2, Loureiro A3, Azoia NG3, Gomes A4, Cavaco-Paulo A3, Gedanken A2 and Gruzman A2

1Department of Chemistry, University of Cambridge, Lensfield road, Cambridge, CB2 1EW, UK

2Department of Chemistry, Bar-Ilan University, Ramat-Gan 52900, Israel

3Center of Biological Enginnering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

4Center of Molecular and Environmental Biology, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

*Corresponding Author:
Shimanovich U
Department of Chemistry, University of Cambridge
Lensfield road, Cambridge, CB2 1EW, UK
Tel: 44-1223-336300, extn 63845
E-mail: [email protected]

Received Date: April 01, 2014; Accepted Date: June 05, 2014; Published Date: June 10, 2014

Citation: Shimanovich U, Munder A, Loureiro A, Azoia NG, Gomes A, et al. (2014) Gene Silencing by siRNA Nanoparticles Synthesized via Sonochemical Method. J Nanomed Nanotechnol 5:204. doi:10.4172/2157-7439.1000204

Copyright: © 2014 Shimanovich U, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The knowledge that small RNAs can affect gene expression has had a tremendous impact on basic and applied research, and gene silencing is currently one of the most promising new approaches for disease therapy. However, RNAs cannot easily penetrate cell membranes, therefore RNA delivery become one of the major challenges for gene silencing technology. In the current paper we discuss a general approach for converting siRNA molecules into a dense siRNA nanoparticles using environmentally friendly sonochemical method. The RNA nanoparticulation enhance its gene-silencing activity in vascular bovine endothelial as well as in cancer 293T/GFP-Puro cell lines without causing any toxic effect. We show that ultrasonic waves do not lead to RNA degradation or any changes in its chemical structure. Moreover, sonochemically produced siRNA nanoparticles have been shown to be resistant to a variety of environmental stresses including pH levels, enzymes and temperatures, hence solving problem of the short half-life of the RNA molecules. As the siRNA nanoparticles are biocompatibile and biodegradabile, and their RNA release properties may be controlled within limits, sonochemical formation of siRNA nanoparticles represent a new promising approach for generation of functional bionano materials.

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