GLAD-PCR Assay of DNA Methylation Markers Associated with Colorectal CancerAlexey A Evdokimov1, Nina A Netesova1, Natalia A Smetannikova1, Murat A Abdurashitov1*, Alexandr G Akishev1, Boris S Malyshev1, Evgeniya S Davidovich1, Vladimir V Fedotov1, Vitaliy V Kuznetsov1, Yuriy D Ermolaev2, Andrey B Karpov2, Alexey E Sazonov2, Ravil M Tahauov2 and Sergey Kh Degtyarev1
- *Corresponding Author:
- Abdurashitov MA
SibEnzyme, 2/12 Ak.Timakov Str.
Novosibirsk 630117, Russia
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Received Date: August 04, 2016; Accepted Date: September 01, 2016; Published Date: September 08, 2016
Citation: Evdokimov AA, Netesova NA, Smetannikova NA, Abdurashitov MA, Akishev AG, et al. (2016) GLAD-PCR Assay of DNA Methylation Markers Associated with Colorectal Cancer. Biol Med (Aligarh) 8: 342. doi:10.4172/0974-8369.1000342
Copyright: © 2016 Evdokimov et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Hypermethylation of the gene regulatory regions is documented for many cancer diseases. Such an aberrant DNA methylation in cancer cells is catalyzed by DNA methyltransferases Dnmt3a and Dnmt3b, which predominantly recognize and methylate RCGY sequences with formation of R(5mC)GY sites. Recently, based on a new methyl-directed DNA endonuclease GlaI, we developed a GLAD-PCR assay, which allows determining R(5mC)GY site in a defi ned position of the genomic DNA. In this work we applied GLAD-PCR assay for identifi cation of the methylated RCGY sites in the regulatory regions of some downregulated genes associated with colorectal cancer (CRC). This list includes ADHFE1, ALX4, CNRIP1, EID3, ELMO1, ESR1, FBN1, HLTF, LAMA1, NEUROG1, NGFR, RARB, RXRG, RYR2, SDC2, SEPT9, SFRP2, SOCS3, SOX17, THBD, TMEFF2, UCHL1, and VIM genes. GLAD-PCR analysis of selected RCGY sites within the regulatory regions of some of these genes demonstrates a good prognostic potential with relatively high sensitivity and specifi city of CRC detection in tumor DNA.