High-Throughput Injection System for Zebrafish Fertilized EggsEriko Avşar-Ban1, Hideo Miyake1, Masaru Obata2, Masatoshi Hashimoto2 and Yutaka Tamaru1*
- *Corresponding Author:
- Yutaka Tamaru
Graduate School of Bioresources
Mie University, 1577 Kurimamachiya
Tsu, Mie 514-8507, Japan
Tel/Fax: +81 59 231 9560
Received date: May 20, 2013; Accepted date: July 25, 2013; Published date: July 31, 2013
Citation: Avsar-Ban E, Miyake H, Obata M, Hashimoto M, Tamaru Y (2013) High-Throughput Injection System for Zebrafish Fertilized Eggs. J Data Mining Genomics Proteomics 4:137. doi:10.4172/2153-0602.1000137
Copyright: © 2013 Avsar-Ban E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source and source are credited.
The zebrafish have been successfully become a popular vertebrate model for studying gene function by large-scale genetic screens due to the see-through embryos and their accessibility to various experimental techniques throughout development. Furthermore, whole genomic sequence of the zebrafish genome has completely finished and its relationship to the human genomic data became clearer. The request of technological development in experiment using zebrafish must be accelerated with the increasing demand of zebrafish for understanding of the roles of specific genes involving human diseases. Here, we introduced a high-throughput injection system for zebrafish fertilized eggs to overcome the problems caused by manual injection and proposed a unique utilization of zebrafish as a host vector system of protein expression, not as a vertebrate models.