alexa High-Throughput Injection System for Zebrafish Fertilized Eggs
ISSN: 2153-0602

Journal of Data Mining in Genomics & Proteomics
Open Access

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Research Article

High-Throughput Injection System for Zebrafish Fertilized Eggs

Eriko Avşar-Ban1, Hideo Miyake1, Masaru Obata2, Masatoshi Hashimoto2 and Yutaka Tamaru1*

1Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan

2Hashimoto Electronic Industry Co., Ltd. 3866-12 Takasu, Matsusaka, Mie 510-0104, Japan

*Corresponding Author:
Yutaka Tamaru
Graduate School of Bioresources
Mie University, 1577 Kurimamachiya
Tsu, Mie 514-8507, Japan
Tel/Fax: +81 59 231 9560
E-mail: ytamaru@bio.mie-u.ac.jp

Received date: May 20, 2013; Accepted date: July 25, 2013; Published date: July 31, 2013

Citation: Avsar-Ban E, Miyake H, Obata M, Hashimoto M, Tamaru Y (2013) High-Throughput Injection System for Zebrafish Fertilized Eggs. J Data Mining Genomics Proteomics 4:137. doi:10.4172/2153-0602.1000137

Copyright: © 2013 Avsar-Ban E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source and source are credited.

 

Abstract

The zebrafish have been successfully become a popular vertebrate model for studying gene function by large-scale genetic screens due to the see-through embryos and their accessibility to various experimental techniques throughout development. Furthermore, whole genomic sequence of the zebrafish genome has completely finished and its relationship to the human genomic data became clearer. The request of technological development in experiment using zebrafish must be accelerated with the increasing demand of zebrafish for understanding of the roles of specific genes involving human diseases. Here, we introduced a high-throughput injection system for zebrafish fertilized eggs to overcome the problems caused by manual injection and proposed a unique utilization of zebrafish as a host vector system of protein expression, not as a vertebrate models.

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