alexa Immunogenicity of Leptospira interrogans Outer Membrane
ISSN: 2157-7560

Journal of Vaccines & Vaccination
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Immunogenicity of Leptospira interrogans Outer Membrane Vesicles in a Hamster Model

Anthicha Kunjantarachot1,3, Weiwei Yan1, Sean P. McDonough2, Siriwan Prapong3, Gunjana Theeragool3 and Yung-Fu Chang1*
1Department of Population Medicine and Diagnostic Sciences, Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, 14853, USA
2Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, 14853, USA
3The Interdisciplinary Graduate Program in Genetic Engineering, the Graduate School, Kasetsart University, Bangkok 10900, Thailand
Corresponding Author : Yung-Fu Chang
Department of Population Medicine and Diagnostic Sciences
College of Veterinary Medicine
Cornell University, Ithaca, NY 14853, USA
Tel: (607)253-3675
Fax: (607)253-3083
E-mail: [email protected]
Received May 06 2014; Accepted June 24 2014; Published June 27 2014
Citation: Kunjantarachot A, Yan W, McDonough SP, Prapong S, Theeragool G, et al. (2014) Immunogenicity of Leptospira interrogans Outer Membrane Vesicles in a Hamster Model . J Vaccines Vaccin 5:239. doi: 10.4172/2157-7560.1000239
Copyright: © 2014 Kunjantarachot A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Animal Leptospirosis, caused by Leptospira spp., is one of the most common zoonotic diseases in the US and throughout the world. Commercially available bacterins are only partially efficacious in that they protect against death, but not against disease. Protective immunity to Leptospira spp. require antibodies specific to outer surface proteins and/or adhesins of leptospires. Spirochetes produce membrane blebs or vesicles (OMVs) and OMVs have been shown to be good immunogens. In this study, we characterized leptospiral OMV components by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of and identified that the majority (58.1%) of proteins in the vesicles were cytoplasmic proteins (294 of 506), while 5 were extracellular proteins (0.99%), 11 were outer membrane proteins (2.17%), 14 were periplasmic proteins (2.77%), 48 were cytoplasmic/inner membrane proteins (9.49%) and 134 were unknown or having multiple locations (26.48%). Transmission electron microscopy (TEM) imaging showed OMVs are spherical bodies with a diameter of 50-200 nm. Vesicles were used to vaccinate hamsters. The results indicated that immunization with Leptospira OMVs induced significant protection against lethal challenge revealed by an enhanced humoral immune response, high survival rate and significantly reduced bacterial burden, all of which were reflected in decreased pulmonary, hepatic and renal lesions (p<0.05). To the best of our knowledge, this is the first report showing that OMVs could be used as a novel vaccine formulation to protect hamsters against lethal challenge.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version