Journal of Plant Pathology & Microbiology

Abstract

Inductions of Defense Response in Tomato against Fusarium Wilt through Inorganic Chemicals as Inducers

NK Pandey, SK Biswas, Mohd Rajik, Rajendra Prasad, Kartikay Bisen and Mohd Shahid

Pre-foliar spray with indole acetic acid, metalaxyl, dipotassium hydrogen orthophosphate, hydrogen peroxide, calcium chloride, salicylic acid and ferric chloride as inducers provided induced resistance in plant against F. o. f.sp. lycopersici, resulting in a decline in the disease incidence from 90.96 to 9.30% after 15 days of pathogen inoculation. The minimum disease incidence (9.30%) was reported from calcium chloride treated plants. Challenge inoculation with abiotic inducers sensitized the seedling to produce increased level of soluble protein. The maximum increase of soluble protein content was found in calcium chloride treated plant showing 35.93, 36.27 and 35.22 mg/g of fresh leaves at 5, 10 and 15 days of pathogen inoculation. Similarly, phenol content was also found to be maximum in calcium chloride treated plant, representing 2.45, 2.76 and 2.67 mg/g of fresh leaves at 5, 10 and 15 days of inoculation. Correlation coefficient analysis revealed that there was negative correlation (r = -0.6214, -0.5867 and -0.5484) between disease severity and soluble protein content after 5, 10 and 15 days of treatment. Similarly, total phenol content also showed negative correlation (r = -0.5370, -0.5656 and -0.4225) with disease incidence. Protein profiling by SDS-PAGE revealed that pre-foliar spray with calcium chloride synthesizes new proteins representing maximum number of 14 protein bands. The genomic DNA was further investigated using 5 universal ITS primers alone and in combination with each other as forward and reverse of Genei series showing all monomorphic bands at 350 bp as compared to 100 bp DNA ladder marker. All the treatments from T1 to T8 of tomato variety, Azad T6 shows the unique identification of combination with ITS primer 1and 4.