alexa Intrauterine Subclinical Inflammation Sensitizes Hypoxic Ischemia- Induced Injury in the Immature Rat Brain and the Mechanisms
ISSN: 2167-0897

Journal of Neonatal Biology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Intrauterine Subclinical Inflammation Sensitizes Hypoxic Ischemia- Induced Injury in the Immature Rat Brain and the Mechanisms

Xu Fa-lin1,2*, Zhang Yan-hua1,2 and Guo Jia-jia1,2
1Department of Pediatrics, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
2Henan Provincial Key Laboratory of Neonatal Brain Injury, Zhengzhou 450052, China
Corresponding Author : Xu Fa-lin
Department of Pediatrics
Third Affiliated Hospital of Zhengzhou University
Zhengzhou 450052, P.R China
Tel: 0371-6690313
E-mail: [email protected]
Received Janaury 09, 2014; Accepted February 04, 2014; Published February 06, 2014
Citation: Fa-lin X, Yan-hua Z, Jia-jia G (2014) Intrauterine Subclinical Inflammation Sensitizes Hypoxic Ischemia-Induced Injury in the Immature Rat Brain and the Mechanisms. J Neonatal Biol 3:126. doi:10.4172/2167-0889.1000126
Copyright: © 2014 Fa-lin X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Objective: To investigate whether intrauterine subclinical infection sensitizes HI-induced brain injury in the immature rat, the changes and significance of Histone deacetylases (HDACs) in brain injury, and the effects of erythropoietin on White Matter injury.
Methods: Pregnant SD rats at gestation day 15 were injected LPS (0.3 mg/kg) or sterile saline (N.S) intraperitoneally, continue to rise until delivery. Rat pups on postnatal (P) days 5 were randomly assigned into 4 groups: control group, LPS, HI, LPS+HI group. Intervention groups included LPS+HI+N.S group and LPS+HI+EPO group. Brain tissues were observed on the time point of 6 h, 24 h and 7d after 40-minHI. The expression of TNF-α and HDACs in the brain homogenate were measured by ELISA. The level of MBP and MAP-2 were detected by immunohistochemistry staining. The expression of MAP-2 mRNA and HDAC1 mRNA were detected by real time PCR.
Results: The expression of TNF-a, HDACs and HDAC1 mRNA from high to low were LPS+HI, LPS/HI, control group, but MBP was the lowest, there were significant differences between LPS+HI group and the other three groups (P<0.05), there were no difference between the other three groups (P>0.05). The expression of MBP in LPS+HI+EPO was higher than LPS+HI+N.S, the difference was statistically significant (P<0.05). There exist necrosis areas in the cortex of LPS+HI group in MAP-2 immunohistochemistry staining, but not in other three groups. Compared with the other three groups, the expression of MAP-2 mRNA in LPS +HI group decreased 6h after HI, and gradually rise, the differences were statistically significant.
Conclusions: Intrauterine subclinical inflammation sensitizes HI-Induced injury in the immature rat brain, and lead to epigenetic changes. EPO plays a protective role to white matter after brain damage.

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords