Investigation of Fusarium verticillioides on the Basis of RAPD Analysis, and Vegetative Compatibility in Iran
- *Corresponding Author:
- Zeynab Bahmani
Department of Plant Pathology
Shahid Chamran University of Ahwaz-61357-831351, Iran
E-mail: [email protected]
Received date: June 26, 2012; Accepted date: November 22, 2012; Published date: November 26, 2012
Citation: Bahmani Z, Nejad RF, Nourollahi K, Fayazi F, Mahinpo V (2012) Investigation of Fusarium verticillioides on the Basis of RAPD Analysis, and Vegetative Compatibility in Iran. J Plant Pathol Microb 3:147. doi:10.4172/2157-7471.1000147
Copyright: © 2013 Jung YG. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Genetic diversity among 41 isolates was determined using vegetative compatibility groups from different
regions of Fars (Firozabad, Ramjerd, Drodzan, Shiraz, Pasargad, Sepidan and Mravdasht) and Khuzestan (Ezh, Ramhormoz and Ahodasht) provinces. Nitrate non-utilizing (Nit) mutants were generated on Czapeck media, containing 5% KClO3. Phenotypic classes of nit mutants were determined to 17 different Vegetative Compatibility Groups (VCGs) on media containing nitrate, hypoxanthine and ammonium. The largest group contained 18 isolates and others contained two and one, respectively. 24 isolates were selected for RAPD test, to represent 17 vegetative compatibility groups. A set of seven random primers revealed a total of 36 alleles. High level of genetic diversity was observed among F. verticillioides isolated in the region. 29 alleles (80.55) showed high polymorphism in all the isolates. Genetic relatedness was calculated based on genetic distance matrix, and 11 groups were clustered based on UPGMA and Dice coefficient.