Lipid Induction in Dunaliella salina Culture Aerated with Various Levels CO2 and Its Biodiesel Production
- *Corresponding Author:
- Hanaa H. Abd El-Baky
Plant Biochemistry Department
National Research Centre
Dokki, Cairo, Egypt
E-mail: [email protected]
Received Date: December 21, 2013; Accepted Date: February 21, 2014; Published Date: February 27, 2014
Citation: Abd El Baky HH, El-Baroty GS, Bouaid A (2014) Lipid Induction in Dunaliella salina Culture Aerated with Various Levels CO2 and Its Biodiesel Production. J Aquac Res Development 5:223 doi:10.4172/2155-9546.1000223
Copyright: © 2014 Abd El Baky HH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this work, the effect of various levels of CO2 (0.01, 0.03, 3.0, 9.0 and 12.0%) aeration on the biomass
production, lipid accumulation and its fatty acid profile as well as biodiesel properties of marine microalgae Dunaliella salina were investigated. The results show that the maximal biomass and lipid productivity (in parenthesis) in cultures aerated with different levels of 0.01, 0.03, 3.0, 9.0 and 12.0% CO2 were 255 (5.36), 412 (15.10), 781 (25.32), 1451 (41.96) and 951 mg/L (59.23 mg L-1d-1), respectively. Whereas, the lipid contents in cells were 2.33, 5.62, 10.28, 28.36 and 40.65%, respectively. Moreover, the levels of CO2 in culture medium had significant effect on fatty acid composition of D. salina. Linolenic and palmitic acids were identified as the major fatty acids in D. salina cells grown at different levels of CO2. The quality of biodiesel produced from algal lipid by a transesterification reaction was located between the limit imposed by the European Standards (EU 14214) and ASTM (US D6751). Based on the results obtained, D. salina could be used for mass-cultured in outdoor ponds, as a promising alternative to current CO2 mitigation strategy and as a suitable feedstock for biodiesel production.