alexa Mechanism of the Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as a Non-Viral Gene Carrier. II. Its Thixotropy Property
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Mechanism of the Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as a Non-Viral Gene Carrier. II. Its Thixotropy Property

Yuki Eshita1*, Junko Higashihara1, Masayasu Onishi2, Masaaki Mizuno3, Jun Yoshida4, Tomohiko Takasaki5, Hidekatsu Yoshioka6, Naoji Kubota7 and Yasuhiko Onishi2*

1Department of Infectious Disease Control, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu-shi, Oita Prefecture 879-5593, Japan

2Ryujyu Science Corporation, 39-4 Kosora-cho, Seto-shi, Aichi Prefecture 489-0842, Japan

3The Center for Genetic and Regenerative Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya-shi, Aichi Prefecture 466- 8550, Japan

4Department of Neurosurgery, Nagoya University Graduate School of Medicine ,65 Tsurumai-cho, Showa-ku, Nagoya-shi, AichiPrefecture466-8550,Japan

5Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjyuku-ku, Tokyo 162-8640, Japan

6Department of Matrix Medicine, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu-shi, Oita Prefecture 879-5593, Japan

7Department of Chemistry, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu-shi, Oita Prefecture 879-5593, Japan

*Corresponding Author:
Yuki Eshita
Department of Infectious Disease Control
Faculty of Medicine, Oita University, 1-1 Idaigaoka
Hasama-machi, Yufu-shi, Oita Prefecture 879-5593, Japan
Fax: +81-97-586-5701
E-mail: [email protected]

Yasuhiko Onishi
Ryujyu Science Corporation, 39-4 Kosora-cho
Seto-shi, Aichi Prefecture 489-0842, Japan
Fax:+81-561-84-3227
E-mail: [email protected]

Received Date: October 07, 2010; Accepted Date: October 28, 2010; Published Date: December 16, 2010

Citation: Eshita Y, Higashihara J, Onishi M, Mizuno M, Yoshida J, et al. (2011) Mechanism of the Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as a Non-Viral Gene Carrier. II. Its Thixotropy Property. J Nanomedic Nanotechnol 2:105. doi:10.4172/2157-7439.1000105

Copyright: © 2011 Eshita Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

From comparative investigations regarding the efficiency of introducing exogenous genes into cultured cells using DEAE-dextran and DEAE-dextran-MMA (methyl methacrylate ester) graft copolymer (2-diethylaminoethyl-dextranmethyl methacrylate graft copolymer; DDMC) as a nonviral carrier, we have confirmed that the gene transfection efficiency of DDMC is higher than that of DEAE-dextran. Comparative investigations in which DNA encoding luciferase (pGL3 control vector; Promega) was introduced into COS-7 cells derived from African green monkey kidney cells with and without the use of an incubator shaker were also carried out using various concentrations of DDMC. Without an incubator shaker, the transfection efficiency results were reversed, namely that the gene introduction efficiency of DDMC was inferior to that of DEAE-dextran. The aqueous solution of the cationic graft-copolymer displays thixotropic properties, which is why a strong shear stress is needed for it to flow and wet the cells. The reaction between DNA and DDMC is thought to be a Michaelis-Menten type complex formation reaction that can be described by the following equation: Complex amount = K1 (DNA concentration) (DDMC concentration).The complex formation reaction is thought to involve Coulomb forces between DDMC and DNA and is also significantly influenced by hydrogen bonding strength along with hydrophobic bonding strength due to the hydrophobicity of the grafted MMA sections.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords