alexa Molecular Profiling - Fruit Carotenoids Components of Six American Heirloom Tomatoes (Solanum lycopersicum)
ISSN: 2090-2697

Journal of Forensic Biomechanics
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Research Article

Molecular Profiling - Fruit Carotenoids Components of Six American Heirloom Tomatoes (Solanum lycopersicum)

Pek Z 1*, Helyes L1, Gyulai G2, Foshee WG3, Daood HG1,3, Lau J3, Vinogradov Sz4, Bittsanszky A5, Goff W5 and Waters LJr5

1St. István University, Faculty of Agricultural & Environmental Sciences, Institutes of Horticulture, Auburn University, Alabama 36849, USA

2Genetics & Biotechnology, Auburn University, Alabama 36849, USA

3Regional Knowledge Transfer Centre, Auburn University, Alabama 36849, USA

4Department of Horticulture, Auburn University, Alabama 36849, USA

5Plant Protection Institute, CAR, Hungarian Academy of Science, Herman O 15, 1022 Budapest, Hungary

*Corresponding Author:
Pek Z
Faculty of Agriculture and Environmental Sciences
Szent Istvan University, Institute of Horticulture
Pater Karoly u. 1. H-2100 Godollo, Hungary
Tel: +36–28–522 071
E-mail: [email protected]

Received Date: April 21, 2016; Accepted Date: June 04, 2016; Published Date: June 13, 2016

Citation: Pek Z, Helyes L, Gyulai G, Foshee WG, Daood HG, et al. (2016) Molecular Profiling - Fruit Carotenoids Components of Six American Heirloom Tomatoes (Solanum lycopersicum). J Forensic Biomed 7:130. doi:10.4172/2090-2697.1000130

Copyright: © 2016 Pek Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use,distribution and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Fruit pigments of six vine-ripening American heirloom tomatoes (Solanum lycopersicum) were analyzed: the green-ripe ‘Aunt Ruby’s German Green’, the red-ripe ‘Black from Tula’, ‘Cherokee Purple’ and ‘German Johnson Regular Leaf’ and the yellow-ripe ‘Kellogg’s Breakfast’ and ‘Yellow Brandywine Platfoot Strain’ which were grown in Hungary (Godollo). In total, twenty-one type of pigments were determined by Reverse Phase (RP) High- Performance Liquid Chromatography (HPLC): the orange colorations of lutein, β-carotene, β-cryptoxanthin, mutatoxanthin and neoxanthin, the red-orange colorations of lycopene, lycopene-epoxide 1, lycopene-epoxide 2, lycoxanthin, 9-cis-lycopene, 13-cis-lycopene, lycopene-diepoxide 1 and lycopene-diepoxide 2 and the third group of colorations of violaxanthin, neochrome, prolycopene, neurosporene-epoxide, neurosporene, ζ(Zeta)-carotene, ζ- carotene-like, and α(alfa)-cryptoxanthin. Tomato ‘Black from Tula’ showed the highest content of β-carotene (23.56 g kg-1). The highest lycopene content (19.25 g kg-1) was found in the ‘Cherokee Purple’ and an extremely high prolycopene (syn.: tetra-cis-lycopene or all-trans-lycopene) content was found in the two yellow fruited tomatoes of ‘Kellogg’s Breakfast’ and ‘Yellow Brandywine Platfoot Strain’ (100.87 and 70. 99 g kg-1, respectively). Brix indexes did not show significant differences. Based on the results suggestions for growing purposes and further use in metabolomics and molecular and DNA profiling are given.

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