alexa Motile Human Sperm Sorting by an Integrated Microfluidic System
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

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Research Article

Motile Human Sperm Sorting by an Integrated Microfluidic System

Hong-Yuan Huang1,2, Hui-Ting Fu3, Hsin-Yao Tsing3, Hung-Ju Huang2, Chin-Jung Li1 and Da-Jeng Yao3*

1Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Kwei-Shan, Tao-Yuan, Taiwan

2Department of Obstetrics and Gynecology, Chang Gung University and College of Medicine, Kwei-Shan, Tao-Yuan, Taiwan

3Institute of Nano Engineering and MicroSystems, National Tsing Hua University, Hsinchu, Taiwan

*Corresponding Author:
Da-Jeng Yao
Institute of Nano Engineering and MicroSystems
National Tsing Hua University, No. 101, Sec. 2
Kuang-Fu Road, Hsinchu 30013, Taiwan
Tel: +886-3-5715131 ext. 42850
Fax: +886-3-5745454
E-mail: [email protected]

Received Date: April 14, 2014; Accepted Date: May 20, 2014; Published Date: May 25, 2014

Citation: Huang HY, Fu HT, Tsing HY, Huang HJ, Li CJ, et al. (2014) Motile Human Sperm Sorting by an Integrated Microfluidic System. J Nanomed Nanotechnol 5:199. doi:10.4172/2157-7439.1000199

Copyright: © 2014 Huang HY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The integrated microfluidic system consists of laminar stream-based microchannels and flow cytometric analysis to enhance the human sperm motility sorting efficiency. This modified sperm sorter provided a new selection that had advances in resolving traditional sorting problems. In order to design the sorting sorting device, CFDRC simulation was performed to analyze the phenomenon of 3-D flow field. Microscopic examination revealed that sperms with different motility. For the purpose of confirming the real sorting situation, the proportions of living and dead sperm after sorting were readily identified and quantified through use of flow cytometric analysis. The viability results of sample injected from two-outlet and four-outlet chip were 95.2% and 92.2% that presented the higher quality of sorting sperm. As a result, more amounts of living sperms that mean motile sperms were counted in four-outlet channels. With this approach, the characteristics of laminar flow and a novel design were utilized to demonstrate the microfluidic system with high efficiency for sperm motility sorting. Therefore, the optimized microfluidic system provides a good opportunity to use and an inexpensive requirement to select the most appropriate sperms before IVF (in vitro fertilization) process.

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