Multiple House Dust Mite Allergen- Sensitization Profiles in Children with Allergic Asthma
- *Corresponding Author:
- Maricar W. Ching
Department of Biological Sciences
College of Science and Technology Centro Escolar University
1005, Manila, Philippines
E-mail: [email protected]
Received date: April 25, 2014; Accepted date: May 30, 2014; Published date: June 05, 2014
Citation: Yap JMG, Ching MW, Cabanilla CQ, Ramos JDA (2014) Multiple House Dust Mite Allergen- Sensitization Profiles in Children with Allergic Asthma. J Allergy Ther 5:179. doi: 10.4172/2155-6121.1000179
Copyright: © 2014 Yap JMG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Allergic asthma is a common chronic inflammatory disorder which affects populations, with increasing prevalence among children. The risk of developing asthma is uncertain but depends on the interaction of environmental and genetic factors. The role of house dust mite (HDM) allergen exposure in the development of sensitization and asthma remains unclear.
Objective: This study determined the sensitization profiles of common HDM species in a population of asthmatic children, which is essential in the development of highly specific and accurate diagnostic and therapeutic strategies for asthma in children.
Methods: The immunoglobulin E-binding activity of allergens from the HDM species Blomia tropicalis (Bt), Dermatophagoides farina (Df), and Dermatopahagoides pteronyssinus (Dp) were determined in 250 age- and sexmatched paediatric allergic asthma and non-atopic Filipino subjects using Enzyme-linked immunosorbent assay and western blot analysis.
Results: Majority of the allergic asthma patients tested were sensitized with multiple allergens from different HDM species where 33% exhibited sensitizations to any two HDM species and 26% were sensitized with allergens from three HDMs. HDM allergens of different molecular weights bind to IgE in allergic asthma patients tested. In addition a significant correlation was observed between total IgE and HDM-specific IgE level among allergic asthma patients (Bt p-value=0.038; Df p-value=0.045; Dp p-value=0.003), suggesting a significant contribution of dust mite allergens in the up-regulation of total serum IgE levels of allergic asthma patients.
Conclusion: The results obtained in this study suggest that the HDM species Bt, Dp, and Df are important sources of allergens that trigger multiple sensitization in children with allergic asthma in the Filipino population. The incorporation of Bt, Dp, and Df allergens in the panel of diagnostic allergens for HDM allergy and allergic asthma is highly recommended.