Nano-Volume Well Array Chip for Large-Scale Propagation and High-Resolution Analysis of Individual Cancer Stem CellsJenifer Clausell-Tormos1, Maria M. Azevedo1, Irene Miranda-Lorenzo1, Catarina R. Vieira1, Yolanda Sanchez-Ripoll1, Diego Megias2 and Christopher Heeschen1,3*
- *Corresponding Author:
- Christopher Heeschen
Molecular Pathology Programme
Spanish National Cancer Research Centre (CNIO), Madrid, Spain
E-mail: [email protected]
Received Date: February 09, 2014; Accepted Date: March 28, 2014; Published Date: March 31, 2014
Citation: Clausell-Tormos J, Azevedo MM, Lorenzo IM, Vieira CR, Sanchez-Ripoll Y, et al. (2014) Nano-Volume Well Array Chip for Large-Scale Propagation and High- Resolution Analysis of Individual Cancer Stem Cells. J Nanomed Nanotechnol 5:191. doi:10.4172/2157-7439.1000191
Copyright: © 2014 Clausell-Tormos J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Cellular heterogeneity represents an increasingly appreciated aspect for research in life science. To address this issue, we have developed a nano-volume well array chip that allows larger-scale isolation and propagation of single cells. Notably, the chip enables single-cell analysis of freshly isolated primary cells at a high-resolution. With an average height of 130 ± 10 μm and an average diameter of 80 ± 10 μm, each nano-volume well can hold up to 0.4 nL of volume, and is compatible with both adherent as well as 3D suspension cultures. Simultaneous time-lapse imaging of thousands of nano-volume wells allows to monitor cell division, as well as tracking of cell fate, and/or alterations in the microscopic cellular morphology and/or markers expression. To demonstrate its application, we employed the system for propagating and tracking of Cancer Stem Cells (CSCs). CSCs could be monitored over three consecutive days by time-lapse high-resolution imaging at the single-cell level. We could demonstrate that non-CSCs do not dedifferentiate into CSCs, while CSCs were able to give rise to both CSCs and non-CSCs by undergoing symmetric and asymmetric division, respectively. Altogether, we have developed a novel nano-volume well array chip that significantly ameliorates clonal propagation and high-resolution image analysis of rare cells.